We narrowed to 67,638 results for: TOR;

1. pcDNA3.1+-mutTornado-split-nLuc

   Plasmid

   #212707

   Purpose

   Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   mutTornado-CVB3-split-nLuc
   

   Use

   Tags

   Expression

   Mutation

   Deleted the partial 3'ribozyme sequence in T…

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1 Flag Raptor Rheb15

   Plasmid

   #26634

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-Rheb15 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-HF-tetra-com-vector

   Plasmid

   #132555

   Purpose

   Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   High fidelity Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HaloTag-A2A-adenosine receptor

   Plasmid

   #66995

   Purpose

   A2A-adenosine receptor tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-A2A-adenosine receptor (ADORA2A Human)
   

   Use

   Tags

   Human Growth hormone Signal Sequence- HaloTag

   Expression

   Mammalian

   Mutation

   The signal sequence from human growth hormone was…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-MTOR-C1483F

   Plasmid

   #69008

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-C1483F (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Cysteine 1483 to Phenylalanine

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Flag-APEX3-C Cloning Vector

   Plasmid

   #220615

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Flag-APEX3 fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+N-3xFlag Cloning Vector

   Plasmid

   #196651

   Purpose

   N-terminal 3xFlag mammalian expression plasmid

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GABA(A) receptor subunit B3SE

   Plasmid

   #49171

   Purpose

   pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

   Depositor

   Stephen Moss, Tija Jacob

   Article

   Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

   Insert

   GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
   

   Use

   Tags

   myc, pHGFP (pHluorin), and thrombin cleavage site…

   Expression

   Mammalian

   Mutation

   GTG (V) to TGC (C)

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Burden Monitor lambda version

   Plasmid

   #66073

   Purpose

   The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. cacnb2a (rat) in pMT2 vector

    Plasmid

    #107424

    Purpose

    Expresses Calcium channel beta2a auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

    Insert

    calcium channel beta2a auxillary subunit (Cacnb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sth1Cas9n cytosine base editor

    Plasmid

    #194209

    Purpose

    Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    Sth1Cas9-D9A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartate 9 to Alanine.

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. myc mTOR kinase dead

    Plasmid

    #8482

    Depositor

    David Sabatini

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D2357E V2364I

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. Circular guide cloning vector

    Plasmid

    #180184

    Purpose

    Cloning vector for expressing circular RNA from a U6 promoter

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GABA(A) receptor subunit a2SE

    Plasmid

    #49169

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Stephen Moss, Tija Jacob

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    

    Use

    Tags

    HA, bovine alpha 1 signal sequence, and pHGFP (pH…

    Expression

    Mammalian

    Mutation

    T343A

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Wild-type AdPol vector

    Plasmid

    #131685

    Purpose

    A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus DNA polymerase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. myc-mTOR (1750-2549 aa)

    Plasmid

    #21747

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1750-2549) (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1750-2549

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-FLAG-MTOR-I2500F

    Plasmid

    #69014

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-I2500F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Isoleucine 2500 to Phenylalanine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5 FLAG human DEPTOR

    Plasmid

    #21334

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N204S and S389N mutations (numbering according to…

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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