We narrowed to 853 results for: Tg

Showing: 461 - 480 of 853 results

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Control-RB1-LRG-GFP

Plasmid

#225875

Purpose

Guides targeting RB1 and a control sequence

Depositor

Janai Carr-Ascher

Article

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

Control and RB1 (RB1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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RNAseq_cal_Tuschl

Plasmid

#59426

Purpose

Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

Depositor

Thomas Tuschl

Article

Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

Insert

RNAseq_cal_Tuschl

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTR-177 pUC19-CLTA-N-mCherry

Plasmid

#186128

Purpose

knockin of mCherry to CLTA target

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CLTA (CLTA Human)

Use

CRISPR

Mutation

WT with mCherry sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-373 tNGFR-P2A-IL7R

Plasmid

#186058

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL7R (IL7R Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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ACTIN-Cxcl5-PGK-Cre

Plasmid

#110278

Purpose

Bicistronic lentivirus expressing Cxcl5 and Cre

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Inserts

chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
cyclization recombinase

Use

Cre/Lox and Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

B-actin and Pgk

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only
AAV2_hSyn_iChloC_CA_Citrine

Plasmid

#98220

Purpose

Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

Depositor

Simon Wiegert

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct iChloC_C128A gene

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

E83Q, E90R, E101S, C128A, T159C, D156N

Promoter

human synapsin

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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KBB430

Plasmid

#185091

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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KBB439

Plasmid

#185093

Purpose

NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

GAL1::NMA111-GFP

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB394

Plasmid

#185086

Purpose

NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

Nma111 KKR 9-11 AAA; KRK 28-30 AAA

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET41a iGluSnFR E25R

Plasmid

#119831

Purpose

Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25R

Tags

GST

Expression

Bacterial

Mutation

E25R

Promoter

T7

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET41a iGlum

Plasmid

#119829

Purpose

Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlum

Tags

GST

Expression

Bacterial

Mutation

R24K

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pIG-721 CTLA4-GFP T124P

Plasmid

#186126

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

T124P

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-703pUC19-tNGFR-P2A-BACH2

Plasmid

#186112

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BACH2 (BACH2 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-340 pUC19-tNGFR-P2A-IL2RG N

Plasmid

#186067

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RG (IL2RG Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-374 tNGFR-P2A-B2M

Plasmid

#186068

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

B2M (B2M Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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PIG-MycT58A

Plasmid

#177648

Purpose

Expression of mouse Myc with T58A point mutation

Depositor

Trudy Oliver

Article

Mollaoglu et al Cancer Cell. 2017 Feb 13;31(2):270-285.

Insert

Myc-T58A (Myc Mouse)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Mutation

T58A point mutation

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-701pUC19-tNGFR-P2A-FOXP3

Plasmid

#186114

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

FOXP3 (FOXP3 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-648 CTLA4int1-CTLA4-GFP-SV40

Plasmid

#186123

Purpose

CTLA4 gene knockin

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

WT with GFP sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-747pUC19-tNGFR-P2A-CD7

Plasmid

#186086

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD7 (CD7 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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