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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,553 results for: golden gate

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Showing: 461 - 480 of 5553 results

  1. pMA-SpCas9-g7

    Plasmid
    #80790
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80790-plasmid-map-sequence-id-161640

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  2. pMA-SpCas9-g6

    Plasmid
    #80789
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80789-plasmid-map-sequence-id-161638

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  8. pOPS0359

    Plasmid
    #115508
    Purpose
    Reporter Plasmid. Taurine-inducible expression of GFP
    Depositor
    Philip Poole
    Publication
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Taurine-inducible promoter of S. meliloti Promote…
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115508-plasmid-map-sequence-id-224277

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  10. pICSL80004

    Plasmid
    #50325
    Depositor
    Nicola Patron
    Publication
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
    Use
    Plant expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50325-plasmid-map-sequence-id-497530

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  16. pNDGG057

    Plasmid
    #231327
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pNDGG005, pOGG012, and pOGG014, NmR/KmR
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231327-plasmid-map-sequence-id-472017

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  17. pNDGG070

    Plasmid
    #231328
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Sucrose curable broad host range plasmid . L1-pBBR1-Sp-sacB-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pNDGG008, and pOGG014, SpR
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231328-plasmid-map-sequence-id-466831

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  18. pNDGG053

    Plasmid
    #231322
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmR
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231322-plasmid-map-sequence-id-462969

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Showing: 461 - 480 of 5553 results