We narrowed to 6,235 results for: golden gate
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Plasmid#113996PurposeSC module. Terminator for T7RNAPDepositorInsertTerminator for T7RNAP
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
BsaI-tagRFP-BsaI-pUC-kan
Plasmid#170771Purposetest plasmid for Golden Gate assembly, encodes fluorescent red proteinDepositorInserttagRFP
PromoternoneAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB1532H-RFP
Plasmid#101855PurposeGolden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCB1532B-RFP
Plasmid#101854PurposeGolden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NHSTIIT-AVITAG-GG
Plasmid#215802PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAGDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-TolB-NHSTIIT-GG
Plasmid#215792PurposeCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-PelB-NH10T-AVITAG-GG
Plasmid#215793PurposeCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-GST-TEV-AVITAG-GG
Plasmid#215809PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOGG083
Plasmid#114001PurposeSC module. Beta-glucosidase reporterDepositorInsertgusA
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC-OmpA-NHSTIIT-GG
Plasmid#215790PurposeCloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-SufI-NHSTIIT-GG
Plasmid#215791PurposeCloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-SUMO1-AVITAG-GG
Plasmid#215808PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pND005
Plasmid#200951PurposeYeast two-hybrid destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast two-hybridPromoterGAL4Available SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_4'_+nls-mCherry-Flag-_+1_BK+
Plasmid#49313PurposeGolden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codonDepositorInsertnls-mCherry-Flag
UseCloning vectorAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_2_+nls-eGFP-HA-_+1_BK+
Plasmid#49312PurposeGolden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codonDepositorInsertnls-EGFP-HA
UseCloning vectorAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
Tags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA-MsgRNA-EGFP
Plasmid#80794PurposeFor insertion of gRNA array containing 11-30 gRNA modulesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEXY (pDN137)
Plasmid#72656Purposegolden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)DepositorTypeEmpty backboneUseGolden gate entry vectorTagsmCherry-LEXYExpressionMammalianPromoterCMV-FAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
GGE0029
Plasmid#120730PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assemblyDepositorTypeEmpty backboneUseCloning/assembly plasmidAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only