Skip to main content
Addgene
Showing: 461 - 480 of 1124 results

  1. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200421-plasmid-map-sequence-id-405458

    Enlarge

  2. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

    Enlarge

  4. pOpen-Endonuclease VIII E. coli

    Plasmid
    #165561
    Purpose
    Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease VIII E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165561-plasmid-map-sequence-id-339954

    Enlarge

  5. p203 Dync1li1 N235Y

    Plasmid
    #26451
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 N235Y (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Tags
    Expression
    Mutation
    changed Asparagine 235 to Tyrosine
    Promoter
    Available since
    Dec. 2, 2010
    Availability
    Academic Institutions and Nonprofits only

  6. pCHA_SBV_NSs

    Plasmid
    #208938
    Purpose
    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 47 bp at 5' end and 510 bp from 3&#x…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208938-plasmid-map-sequence-id-419026

    Enlarge

  7. pET28-Flag-RBM24-95~236

    Plasmid
    #208634
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 95~236, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208634-plasmid-map-sequence-id-418349

    Enlarge

  8. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

    Enlarge

  14. pIA035

    Plasmid
    #200426
    Purpose
    amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200426-plasmid-map-sequence-id-405336

    Enlarge

  15. pIA027

    Plasmid
    #200418
    Purpose
    amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200418-plasmid-map-sequence-id-405455

    Enlarge

  16. pGL3-pSMANTIS_1kb_1 KLF2mut 449,899

    Plasmid
    #194198
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,899)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194198-plasmid-map-sequence-id-381969

    Enlarge

  17. pGL3-pSMANTIS_1kb_1 KLF2mut 449,548

    Plasmid
    #194199
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,548)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194199-plasmid-map-sequence-id-381923

    Enlarge

  18. pGL3-pSMANTIS_1kb_1 KLF2mut 449,732

    Plasmid
    #194200
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194200-plasmid-map-sequence-id-381961

    Enlarge

  19. pGL3-pSMANTIS_1kb_1 KLF2mut 449,884

    Plasmid
    #194201
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,884)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194201-plasmid-map-sequence-id-381985

    Enlarge

Showing: 461 - 480 of 1124 results