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Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
UseTagsExpressionBacterialMutationGvpA T6A-I48VPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca0.1(Na0.1, 143-144)
Plasmid#198599PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-143-144
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), N229A (EcQ) MetNIQ in pBAD
Plasmid#118581PurposeN295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala) and…PromoterNone and araBAD PromotoerAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), Y160A (EcI) MetNIQ in pBAD
Plasmid#118260PurposeN295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala) …PromoterNone and araBAD PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
UseTagsExpressionBacterialMutationGvpA T6A-L40APromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), M163A (EcI) MetNIQ in pBAD
Plasmid#118261PurposeN295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala) …PromoterNone and araBAD PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), M107A (EcI) MetNIQ in pBAD
Plasmid#118259PurposeN295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala) …PromoterNone and araBAD PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), F103A (EcI) MetNIQ in pBAD
Plasmid#118258PurposeN295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala) …PromoterNone and araBAD PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZa pBAD csgA CBDcex pLac Ara csgGEF
Plasmid#226378PurposePlasmid carries csgA and csgG,E,F genes of curli operonDepositorInsertcsgA and csgG,E,F genes of curli operon
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
Plasmid#110547PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.DepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A, H840APromoterpBADAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015
Plasmid#202025PurposeExpresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coliDepositorInsertBacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
UseTagsExpressionBacterialMutationGvpB S9G-R31L-R85LPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA, araBAD and LacO3O1 promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500
Plasmid#45788DepositorInsertdeGFP
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterpBAD-tetO1Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKPY115
Plasmid#72669PurposeEncodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) ControlDepositorInsertThr251Gly-EcPheRS
UseTagsExpressionBacterialMutationThr251Gly in Alpha SubunitPromoterPBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPARA1
Plasmid#200416PurposeGolden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPARA2
Plasmid#200417PurposeGolden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWT018a
Plasmid#107885PurposeR3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT004a
Plasmid#107883PurposeR1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1029
Plasmid#22066DepositorTypeEmpty backboneUseTagsT25-FlagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEB1030
Plasmid#22067DepositorTypeEmpty backboneUseTagsT18-FlagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only