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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Mac-GFP

Plasmid

#58803

Purpose

AAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Mac-GFP

Plasmid

#58803

Purpose

AAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pOttc1473 - pAAV CaMKII KDELR2-Myc-DDK

Plasmid

#192600

Purpose

An AAV packaging vector that expresses KDELR2 under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

KDELR2 (KDELR2 Human)

Use

AAV

Promoter

CaMKII

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pOttc1473 - pAAV CaMKII KDELR2-Myc-DDK

Plasmid

#192600

Purpose

An AAV packaging vector that expresses KDELR2 under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

KDELR2 (KDELR2 Human)

Use

AAV

Promoter

CaMKII

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSaCas9-KRAB(Zim3)-pA

Plasmid

#255030

Purpose

AAV plasmid encoding a dSaCas9-KRAB(Zim3) fusion gene controlled by a CMV promoter

Depositor

Jonathan Ploski

Insert

dSaCas9-KRAB(Zim3)

Use

AAV and CRISPR

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSaCas9-KRAB(Zim3)-pA

Plasmid

#255030

Purpose

AAV plasmid encoding a dSaCas9-KRAB(Zim3) fusion gene controlled by a CMV promoter

Depositor

Jonathan Ploski

Insert

dSaCas9-KRAB(Zim3)

Use

AAV and CRISPR

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-PiGM-Iq(535/81, eGFP)

Plasmid

#221612

Purpose

AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).

Depositor

John Lin

Insert

PiGM-Iq (535/81,eGFP)

Use

AAV

Mutation

RGS2 1-53 truncation

Promoter

Human Synapsin

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-PiGM-Iq(535/81, eGFP)

Plasmid

#221612

Purpose

AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).

Depositor

John Lin

Insert

PiGM-Iq (535/81,eGFP)

Use

AAV

Mutation

RGS2 1-53 truncation

Promoter

Human Synapsin

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph20_eGFP_CCR5TC

Plasmid

#187444

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph20_eGFP_CCR5TC

Plasmid

#187444

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AiP15022 - pAAV-AiE1398m-minBG-SYFP2-P2A-3XFLAG-H2B-WPRE3-BGHpA

Plasmid

#243314

Purpose

AiE1398m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2-P2A-3XFLAG-H2B

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15022 - pAAV-AiE1398m-minBG-SYFP2-P2A-3XFLAG-H2B-WPRE3-BGHpA

Plasmid

#243314

Purpose

AiE1398m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2-P2A-3XFLAG-H2B

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Article

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Article

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM4Di-mCherry

Plasmid

#161579

Purpose

To express hM4Di in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM4Di-mCherry

Plasmid

#161579

Purpose

To express hM4Di in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIα-FLAG-STIM1(238-685)

Plasmid

#248299

Purpose

An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the CaMKIIα promoter.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) (STIM1 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

CamKlla

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIα-FLAG-STIM1(238-685)

Plasmid

#248299

Purpose

An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the CaMKIIα promoter.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) (STIM1 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

CamKlla

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-GfaABC1D-FLAG-STIM1(238-685)

Plasmid

#248301

Purpose

An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the GfaABC1D promoter.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) (STIM1 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

GfaABC1D

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-GfaABC1D-FLAG-STIM1(238-685)

Plasmid

#248301

Purpose

An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) under the control of the GfaABC1D promoter.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238-685) (STIM1 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

GfaABC1D

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

Plasmid

#226935

Purpose

Encoding SaKKHABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSaKKHCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

Plasmid

#226935

Purpose

Encoding SaKKHABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSaKKHCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK1A(K188R)-2A-mCherry

Plasmid

#177166

Purpose

Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

Changed Lysine 188 to Arginine

Promoter

CMV with beta globin intron

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK1A(K188R)-2A-mCherry

Plasmid

#177166

Purpose

Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

Changed Lysine 188 to Arginine

Promoter

CMV with beta globin intron

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pgk-DIO-rvCRY-WPRE

Plasmid

#182060

Purpose

Plasmids for production of AAV-based TFactivity reporter

Depositor

Kentaro Abe

Article

Abe et al iScience. 2022 Feb 14;25(3):103927. doi:

Insert

mCherry

Use

AAV

Promoter

hu Pgk1

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge