We narrowed to 233,224 results for: Gene

1. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Promoter

   Promoterless

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRK892

   Plasmid

   #71803

   Purpose

   Expression of mutated L. gasseri gusA from strong Ppgm promoter

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

   Insert

   gusA3
   

   Expression

   Bacterial

   Mutation

   D573A

   Promoter

   Ppgm

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-puro-2A-TEVp

   Plasmid

   #99610

   Purpose

   Lentivirus expressing puromycin resistance gene and TEV protease

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   Puromycin resistance (PAC gene)
   TEV protease
   

   Use

   Lentiviral

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWJ66

   Plasmid

   #46570

   Purpose

   Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9-w
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gg 3kb Green opsin GFP

   Plasmid

   #72919

   Purpose

   green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

   Depositor

   Joseph Corbo

   Article

   Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

   Insert

   chicken green opsin promoter
   

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL-rpb1-N488D

   Plasmid

   #10988

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-N488D (RPO21 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 1462 A to G in the RPB1 ORF resulting in…

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

10. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Tags

    EE and GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-Cre.zf1

    Plasmid

    #61391

    Purpose

    mRNA synthesis of Cre recombinase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Cre.zf1
    

    Use

    Zebrafish expression

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Flag-Kdm4b-pA

    Plasmid

    #104486

    Purpose

    Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

    Depositor

    Yi Zhang

    Article

    Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

    Insert

    Kdm4b (Kdm4b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOSCAR

    Plasmid

    #29639

    Depositor

    David_L Andrews, Scott Gold

    Article

    Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

    Type

    Empty backbone

    Available Since

    Sept. 19, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCRU5HT1-inLuc

    Plasmid

    #58954

    Purpose

    HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

    Depositor

    David Derse, Dmitriy Mazurov

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Retroviral

    Promoter

    CMV/R/U5

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-M13-TEV-C-P2A-TdTomato

    Plasmid

    #92391

    Purpose

    produces AAV expressing Cal-light M13-TevC component and TdTomato

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    M13-TEV-C-P2A-TdTomato
    

    Use

    AAV and Synthetic Biology

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    pTet

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-HBH

    Plasmid

    #88896

    Purpose

    Carries phiC31 integrase attB site flanked by cHS4 insulators.

    Depositor

    Michele Calos

    Article

    Zhao et al PLoS One. 2014 Apr 29;9(4):e96279. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBPp65ADZpUw

    Plasmid

    #26234

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    p65AD-Zip (RELA Human)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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