We narrowed to 17,420 results for: She

1. pSN667

   Plasmid

   #219963

   Purpose

   Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

   Depositor

   Shinsuke Niwa

   Insert

   codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
   

   Use

   Tags

   sfGFP::2xStrepII tag

   Expression

   Insect

   Mutation

   encodes 1-721

   Promoter

   polyhedrin

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MARS-nGFP

   Plasmid

   #205234

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
   

   Use

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3-2xMARS-PLD

   Plasmid

   #205237

   Purpose

   Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

   Depositor

   Jeremy Baskin

   Inserts

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   phospholipase D (PLD)
   

   Use

   Tags

   Nuclear Export Sequence and mCherry

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSN668

   Plasmid

   #219962

   Purpose

   Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells

   Depositor

   Shinsuke Niwa

   Insert

   codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
   

   Use

   Tags

   sfGFP::2xStrepII tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

   Plasmid

   #214469

   Purpose

   Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TCF4 - P381L in pENTR

   Plasmid

   #216570

   Purpose

   gateway cloning donor vector containing TCF4 ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   P381L

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TCF4 - D424N in pENTR

   Plasmid

   #216571

   Purpose

   gateway cloning donor vector containing TCF4 ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   D424N

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG426-GAL-APP-CTF

   Plasmid

   #181732

   Purpose

   Galactose inducible expression of APP-CTF

   Depositor

   Alejandro Chavez

   Insert

   APP-CTF (APP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C-terminal fragment (aa 671-770)

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PE-SUMO CRYGS Q16C

   Plasmid

   #217674

   Purpose

   For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

   Depositor

   Larry David

   Insert

   CRYGS (CRYGS Human)
   

   Use

   Tags

   6XHis and SUMO

   Expression

   Bacterial

   Mutation

   Glutamine 16 changed to Cysteine

   Promoter

   T7

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #219653

    Purpose

    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

    Depositor

    Thomas Oertner

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

    Expression

    Mammalian

    Mutation

    Promoter

    nEf Addgene # 149296

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-H12TEV-hEIF5B-587~1220

    Plasmid

    #208637

    Purpose

    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MECP2 - S411R in pENTR

    Plasmid

    #216603

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    S411R

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MECP2 - P388S in pENTR

    Plasmid

    #216602

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P388S

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MECP2 - P376S in pENTR

    Plasmid

    #216601

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P376S

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MECP2 - E282G in pENTR

    Plasmid

    #216600

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    E282G

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MECP2 - P217L in pENTR

    Plasmid

    #216599

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P217L

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MECP2 - A202G in pENTR

    Plasmid

    #216598

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    A202G

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MECP2 - G195S in pENTR

    Plasmid

    #216597

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    G195S

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MECP2 - P152L in pENTR

    Plasmid

    #216596

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P152L

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MECP2 - P152R in pENTR

    Plasmid

    #216595

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P152R

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MECP2 - R91W in pENTR

    Plasmid

    #216593

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    R91W

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21b-hHNF4aORF-1

    Plasmid

    #206959

    Purpose

    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRE-tdTomato

    Plasmid

    #210512

    Purpose

    tdTomato reporter vector for LAUNCHER system

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid

    #208626

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 191~307, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid

    #208629

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 212~285, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCW-LIC-N8C_Fab5a

    Plasmid

    #203693

    Purpose

    Expresses N8C_Fab5a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab5a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCW-LIC-N8C_Fab2a

    Plasmid

    #203688

    Purpose

    Expresses N8C_Fab2a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCW-LIC-N8C_Fab1b

    Plasmid

    #203691

    Purpose

    Expresses N8C_Fab1b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202024

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-L40A

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

    Plasmid

    #201187

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 2 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

    Plasmid

    #201188

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 3 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. OA-1067L

    Plasmid

    #200253

    Purpose

    pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    6 gRNAs targeting Doublesex, Intersex, bTublin
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198049

    Purpose

    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pB2AR-miRFP718nano

    Plasmid

    #195751

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    B2AR-miRFP718nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMyosin-miRFP718nano

    Plasmid

    #195748

    Purpose

    LC-Myosin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-LC-Myosin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. VPS13D(∆ATG2-C/PH)^EGFP

    Plasmid

    #194004

    Purpose

    Deletion of DHPH domain of VPS13D

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D (VPS13D Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    early stop codon before DHPH

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBAD/His-miRFP718nano

    Plasmid

    #195743

    Purpose

    Monomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge