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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,649 results for: Pol

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Showing: 4801 - 4820 of 7649 results

  1. pJEC626

    Plasmid
    #174367
    Purpose
    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23106
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174367-plasmid-map-sequence-id-345703

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  3. pLKO.puro_shHuR 3UTR

    Plasmid
    #110414
    Purpose
    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA PolIII)
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110414-plasmid-map-sequence-id-213427

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  5. 6WNX

    Plasmid
    #162270
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    FBXW11 (FBXW11 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Promoter
    Polyhedrin
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162270-plasmid-map-sequence-id-319834

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  11. pOCC230

    Plasmid
    #118871
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mCitrine, cl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118871-plasmid-map-sequence-id-232332

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  12. pOCC69

    Plasmid
    #118874
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mGFP, cleava…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118874-plasmid-map-sequence-id-232330

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  13. pKP4010

    Plasmid
    #113336
    Purpose
    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter
    Depositor
    Suzanne Sandmeyer
    Article
    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do
    Inserts
    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    Tags
    3X-FLAG/8X-Gly linker
    Expression
    Yeast
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113336-plasmid-map-sequence-id-217436

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  14. p3E DN Kif5a

    Plasmid
    #105973
    Purpose
    3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    Kif5a (kif5aa Zebrafish)
    Expression
    Bacterial
    Mutation
    truncation of aa 416-1033
    Available Since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105973-plasmid-map-sequence-id-203068

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  15. 5WCJ (METTL13)

    Plasmid
    #101270
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL13 (METTL13 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Promoter
    polyhedrin
    Available Since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101270-plasmid-map-sequence-id-197531

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  18. KCTD13A

    Plasmid
    #74654
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCTD13A (KCTD13 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 4801 - 4820 of 7649 results