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2_T7-mScarlet3-KRas

Plasmid

#225929

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

Depositor

Michalis Averof

Publication

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

mScarlet3-KRas

Use

In vitro transcription

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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10_T7-SP-CD8tm-mScarlet3

Plasmid

#225937

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

Depositor

Michalis Averof

Publication

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

SP-CD8tm-mScarlet3

Use

In vitro transcription

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AA145

Plasmid

#215937

Purpose

Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10-SNAP-GW

Plasmid

#209350

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

Depositor

Lucia Strader

Publication

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pR540

Plasmid

#207885

Purpose

template for 2 gRNA amplification

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

scaffold-pU6

Expression

Bacterial

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJBJZ_001

Plasmid

#155357

Purpose

Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_003

Plasmid

#155358

Purpose

Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_006

Plasmid

#155359

Purpose

Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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ZS007:eGFP-TM

Plasmid

#186429

Purpose

eGFP-TM

Depositor

Adam Cohen

Publication

Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP-TM

Expression

Mammalian

Promoter

cmv

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTCF3 H-2D(d)

Plasmid

#180452

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2D(d)

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

His

Expression

Insect

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D3

Plasmid

#155216

Purpose

Expresses human Muc2 residues 858-1259

Depositor

Deborah Fass

Publication

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK211

Plasmid

#110595

Purpose

BTK Type 6 Repressor CDS for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

LacI reverse

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBP-Pveg

Plasmid

#112776

Purpose

Strong promoter active during sporulation and vegetative growth

Depositor

Chris French

Publication

Wicke et al bioRxiv 185108

Insert

Pveg promoter

Expression

Bacterial

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Publication

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG34

Plasmid

#26940

Depositor

Geraldine Seydoux

Publication

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

cgh-1 (cgh-1 Nematode)

Tags

GFP

Expression

Worm

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pIF1-GFP

Plasmid

#206925

Purpose

Plasmid expressing human IF1 fused in C-terminal with GFP

Depositor

José Cuezva

Publication

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

human IF1 (ATPase inhibitory factor 1) (ATP5IF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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