We narrowed to 9,802 results for: otos

1. sgMARK3-R-3

   Plasmid

   #229439

   Purpose

   knockout of MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK3-R-3 (MARK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgMARK3-R-1

   Plasmid

   #229437

   Purpose

   knockout of MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK3-R-1 (MARK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgMARK2-R-3

   Plasmid

   #229436

   Purpose

   knockout of MARK2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-R-3 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgMARK2-R-2

   Plasmid

   #229435

   Purpose

   knockout of MARK2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-R-2 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgMARK2-R-1

   Plasmid

   #229434

   Purpose

   knockout of MARK2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-R-1 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-YAP-5D

   Plasmid

   #229415

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   YAP-5D (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   MARK2 phosphosites (S128,S163,S350,T354,T398) mut…

   Promoter

   EFS

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-YAP-5A

   Plasmid

   #229413

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   YAP-5A (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   MARK2 phosphosites (S128,S163,S350,T354,T398) mut…

   Promoter

   EFS

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

   Plasmid

   #224496

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b-ptetO::bac::gfp

   Plasmid

   #217882

   Purpose

   expresses the bac-BGC in bacterial cells, produces bacillamide D

   Depositor

   Tobias A. M. Gulder

   Article

   Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

   Inserts

   Dehydrogenase
   Non-ribosomal peptide synthetase
   Aminotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptetO

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hTph2p-Luc

    Plasmid

    #223526

    Purpose

    Firefly-luciferase reporter expression driven by human Tph2 promoter

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    Tph2 promoter (TPH2 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    hTph2 promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mTph2p(330bp)-Luc

    Plasmid

    #223663

    Purpose

    Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    mTph2 promoter (Tph2 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    mTph2 promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMRXIP-GFP-TRPML1(PHD)

    Plasmid

    #221752

    Purpose

    Stable expression of GFP-TRPML1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TRPML1(PHD) (amino acids 1–69)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIG-LSL-LRRC37B

    Plasmid

    #220791

    Purpose

    To express the human receptor LRRC37B (cre-dependant) in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    LRRC37B (LRRC37B Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCIG-LRRC37B-HA

    Plasmid

    #220792

    Purpose

    To express the human receptor LRRC37B HA-tagged in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    LRRC37B (LRRC37B Human)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psiCHECK-VIM270-pSM

    Plasmid

    #215871

    Purpose

    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

    Depositor

    Kumiko Ui-Tei

    Article

    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

    Insert

    3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK-VIM270-pCM

    Plasmid

    #215869

    Purpose

    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

    Depositor

    Kumiko Ui-Tei

    Article

    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

    Insert

    target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-PARP1-del.p119K120S–eGFP

    Plasmid

    #211554

    Purpose

    A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 119K 120S

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-749_HA-GD2-28z_CAR_TFAP4

    Plasmid

    #207490

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-852_HA-GD2-28z_TFAP4-HA

    Plasmid

    #207502

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIG-858_CD19-BBz_CAR_TFAP4

    Plasmid

    #207503

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-BBz_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-U6gRNA5(ZIC3_5-3)-PGKpuroBFP-W

    Plasmid

    #211996

    Purpose

    Express gRNA against ZIC3 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting ZIC3 (ZIC3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-U6gRNA5(ZIC3_5-5)-PGKpuroBFP-W

    Plasmid

    #211997

    Purpose

    Express gRNA against ZIC3 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting ZIC3 (ZIC3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKLV2-U6gRNA5(RUNX1T1 g3)-PGKpuroBFP-W

    Plasmid

    #211984

    Purpose

    Express gRNA against RUNX1T1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting RUNX1T1 (RUNX1T1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(RUNX1T1 g7-5)-PGKpuroBFP-W

    Plasmid

    #211985

    Purpose

    Express gRNA against RUNX1T1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting RUNX1T1 (RUNX1T1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W

    Plasmid

    #211988

    Purpose

    Express gRNA against TRIM24 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKLV2-U6gRNA5(TRIM24_5-4)-PGKpuroBFP-W

    Plasmid

    #211989

    Purpose

    Express gRNA against TRIM24 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKLV2-U6gRNA5(TRIM25_5-2)-PGKpuroBFP-W

    Plasmid

    #211990

    Purpose

    Express gRNA against TRIM25 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting TRIM25 (TRIM25 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKLV2-U6gRNA5(TRIM25_v3_6-4)-PGKpuroBFP-W

    Plasmid

    #211991

    Purpose

    Express gRNA against TRIM25 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting TRIM25 (TRIM25 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV2-U6gRNA5(NR5A2_5-2)-PGKpuroBFP-W

    Plasmid

    #211976

    Purpose

    Express gRNA against NR5A2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting NR5A2 (NR5A2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge