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AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163476

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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AiP2017 - pAAV-AiE0774m-minBG-iCre(R297T)-BGHpA

Plasmid

#220723

Purpose

AiE0774m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1774 - pAAV-AiE2449m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214540

Purpose

AiE2449m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

Plasmid

#84468

Purpose

pAAV-CRAM-tdTomato

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

T230I (please see depositor comments below)

Promoter

TRE

Available since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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AiP1357 - pAAV-AiE2128m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220643

Purpose

AiE2128m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1287 - pAAV-AiE2103m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214480

Purpose

AiE2103m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1971 - pAAV-AiE2587m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214599

Purpose

AiE2587m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR

Plasmid

#154001

Purpose

Vector expressed CasRx and sgRNA_Ptbp1 for AAV package

Depositor

Hui Yang

Article

Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

Insert

CasRx, Ptbp1 sgRNAs

Use

CRISPR and Mouse Targeting

Tags

FLAG

Expression

Mutation

Promoter

GFAP, U6

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37090

Purpose

Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183529

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Tags

Expression

Mutation

I146M/G174S

Promoter

Ef1a

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Use

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG and TRE

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

pRAM and TRE

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA

Plasmid

#220717

Purpose

AiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1976 - pAAV-AiE2569m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214600

Purpose

AiE2569m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109316

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

eGFP gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA

Plasmid

#154871

Purpose

Human synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrine

Depositor

Ulrik Gether

Insert

KORD-IRES-mCitrine

Use

AAV; Flp-frt

Tags

HA

Expression

Mammalian

Mutation

Promoter

Human Synapsin-1

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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AiP1911 - pAAV-AiE2114m-minBG-iCre-WPRE-BGHpA

Plasmid

#220719

Purpose

AiE2114m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

nEF

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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