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pMCB306

Plasmid

#89360

Purpose

sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Publication

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

GFP, puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDONR223-PAK1

Plasmid

#23543

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK1 (PAK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMSCV-BTK-mCherry

Plasmid

#50043

Purpose

Expression of BTK-mCherry in mammalian cells

Depositor

Hidde Ploegh

Publication

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

BTK (Btk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-ARAF

Plasmid

#23725

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ARAF (ARAF Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGST-RXRalpha

Plasmid

#16547

Depositor

Bert Vogelstein

Publication

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

RXR alpha (RXRA Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-224

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-cRAF-W22

Plasmid

#15270

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CRAF (RAF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C-terminal deletion

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
YFP-DBD

Plasmid

#14874

Depositor

Alexandra Newton

Publication

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DAG binding domain (Prkcb Rat)

Tags

YFP

Expression

Mammalian

Mutation

Y123W

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pQUAST-mCD8-GFP

Plasmid

#24351

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

CD8-GFP-SV40

Expression

Insect

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEZYshuttle

Plasmid

#18703

Purpose

pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system

Depositor

Yu-Zhu Zhang

Publication

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

Plasmid

#37755

Purpose

Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-on

Tags

TdTomato

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PERK 7: PERK.K618A.9E10.pBABE

Plasmid

#21820

Depositor

David Ron

Insert

PERK K618A (Eif2ak3 Mouse)

Use

Retroviral

Tags

Myc (9E10)

Expression

Mammalian

Mutation

kinase dead K618A mutant

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-GFP-ER2

Plasmid

#65016

Purpose

AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

AAV

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3 Flag MKK7b1(glu)

Plasmid

#14673

Depositor

Roger Davis

Publication

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MKK7b1(Glu) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Ser-269Glu Thr-273Glu

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3-T7-Keap1

Plasmid

#21552

Depositor

Yue Xiong

Publication

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Tags

T7

Expression

Mammalian

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pBID-UASC-FG

Plasmid

#35201

Depositor

Brian McCabe

Publication

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Tags

3XFlag

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-MAP3K12

Plasmid

#23626

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K12 (MAP3K12 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183523

Purpose

Optogenetics

Depositor

Karl Deisseroth

Publication

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-CHEK2

Plasmid

#23843

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CHEK2 (CHEK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lyn FAK biosensor

Plasmid

#78299

Purpose

FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lyn Fak biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBABE-puro SHP2 E76A

Plasmid

#8331

Depositor

Ben Neel

Publication

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E76A (activated mutant)

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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