We narrowed to 29,054 results for: nans

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pET21a-hAIM2-deltaPYD

Plasmid

#51522

Purpose

Express His6-tagged HIN domain of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Mutation

deletion of pyrin domain

Promoter

lac operator

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-kinase10

Plasmid

#59123

Purpose

FAK fret control sensor without FERM domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pET21a-hAIM2-PYD-GST

Plasmid

#51531

Purpose

Express GST-tagged PYD of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST and T7

Expression

Bacterial

Mutation

deletion of amino acids 102-343

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS6_4323_v1

Plasmid

#126859

Purpose

4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS6_4323_v1

Use

Synthetic Biology

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-FERM10

Plasmid

#59126

Purpose

FAK fret control sensor without kinase domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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REKAR67

Plasmid

#223261

Purpose

EKAREN4 modified to be a Red-FRET sensor using miRFP670nano3 and miRFP720 (in that order; AKA REKAR67)

Depositor

John Albeck

Article

DeCuzzi et al J Biol Eng. 2025 Nov 13;19(1):102. doi:

Insert

REKAR-67

Tags

miRFP670nano3 and miRFP720

Expression

Bacterial and Mammalian

Mutation

miRFP670nano3 - EKAREN4 - miRFP720

Promoter

hEF-1a

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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REKAR76

Plasmid

#223262

Purpose

EKAREN4 modified to be a Red-FRET sensor using miRFP720 and miRFP670nano3 (in that order; AKA REKAR76)

Depositor

John Albeck

Article

DeCuzzi et al J Biol Eng. 2025 Nov 13;19(1):102. doi:

Insert

REKAR-76

Tags

miRFP670nano3 and miRFP720

Expression

Bacterial and Mammalian

Mutation

miRFP720 - EKAREN4 - miRFP670nano3

Promoter

hEF-1a

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GBKT7

Plasmid

#248655

Purpose

Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 binding domain, Myc

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET32a-Nb19-P4S

Plasmid

#236500

Purpose

Expresses anti-Apolipoprotein A1 nanobody Nb19 as a fusion protein with coiled coil P4S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

Nb19-P4S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-301-P3S

Plasmid

#236498

Purpose

Expresses anti-RiVax nanobody 301 as a fusion protein with coiled coil P3S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

301-P3S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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LSAM domain expression plasmid

Plasmid

#240215

Purpose

Plasmid for the recombinant expression of the LSAM domain of human legumain in E. coli.

Depositor

Elfriede Dall

Article

Dahms et al STAR Protocols, 6, 3, 103991, 19 Septemb

Insert

LSAM (LGMN Human)

Tags

PC1pro

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1376

Plasmid

#239829

Purpose

WT OrufIscB omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB omegaRNA scaffold

Expression

Mammalian

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_mEmerald_I3

Plasmid

#231681

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two tandem mEmeralds in mammalian cells. Assembles into nanocages tagged with 120 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_I3

Plasmid

#231680

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with one mEmerald in mammalian cells. Assembles into nanocages tagged with 60 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20-HA-TEADiv2

Plasmid

#228877

Purpose

Tet-inducible lentiviral vector for expression of optimized 2xHA-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Lentiviral

Tags

2xHA

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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