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pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

Plasmid

#192339

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-EGFR NLS

Plasmid

#192315

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-ERBB2-2xFKBP-HA hygro

Plasmid

#192292

Purpose

Retroviral expression vector for NGF-ERBB2 -2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-ERBB2-2xFKBP-HA (ERBB2 Human)

Use

Retroviral

Tags

HA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FHIP-L

Plasmid

#198528

Purpose

Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FHIP-L (FHIP1A Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH

Plasmid

#148878

Purpose

Mammalian Expression of TNFalpha-ARE-polyA-Malat

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

TNFalpha-ARE-polyA-Malat (TNF Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_571-676-GB1_AG

Plasmid

#148745

Purpose

Bacterial Expression of HseIF4G_571-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_571-676 (EIF4G1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194367

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194368

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194369

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194361

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD

Plasmid

#148519

Purpose

Mammalian Expression of HseIF4G1_615-654-L640AV646A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-L640AV646A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

Plasmid

#148524

Purpose

Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-D830A/E831A

Plasmid

#188085

Purpose

Expresses human ATG9-D830A/E831A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

D830A/E831A

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (801-839)

Plasmid

#188089

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (723-839)

Plasmid

#188088

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (723-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (801-839)

Plasmid

#188093

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (828-839)

Plasmid

#188090

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (828-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-HseIF4E_AF

Plasmid

#148669

Purpose

Bacterial Expression of HseIF4E

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E (EIF4E Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence of i…

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgAgo2_Nterm

Plasmid

#170920

Purpose

Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo2 (Ago2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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