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pMT-GFP-Spindly

Plasmid

#24282

Depositor

Ron Vale

Publication

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Spindly (Spindly Fly)

Tags

GFP

Expression

Insect

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F53

Plasmid

#23077

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F30

Plasmid

#23066

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 9 to Glutamine H3 K9Q

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
UMI-sgRNA-CH

Pooled library

#234820

Purpose

Unique molecular identifiers-linked sgRNA library targeting chromatin factors (CH) in mice.

Depositor

Benjamin Ebert

Publication

Loke et al Blood. 2025 Jun 10:blood.2024028033. doi

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
pRK5_mEGFP-NUP98-HOXA9

Plasmid

#237639

Purpose

For overexpression of mEGFP-NUP98-HOXA9

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NUP98-HOXA9 (NUP98, HOXA9 Human)

Tags

mEGFP

Expression

Mammalian

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -2

Plasmid

#180009

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Publication

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJAK080

Plasmid

#167279

Purpose

CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile

Depositor

Robert Fagan

Publication

Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

Type

Empty backbone

Use

E. coli - c. difficile shuttle vector

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Publication

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Publication

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IKB-alpha

Tags

ECFP

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Publication

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human Rab27B GG (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

Plasmid

#70191

Purpose

Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

Depositor

Richard Murray

Publication

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ClpX (clpX )

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S

Plasmid

#213591

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/D321N

Plasmid

#213598

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cells

Depositor

Channing Der

Publication

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, D321N

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB340_ZFcharm_Kv1_Prnp_DPM

Plasmid

#220843

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding site

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

Plasmid

#199262

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

Depositor

Erik Sontheimer

Publication

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL-C144A

Plasmid

#160287

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL-C143A

Plasmid

#160289

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C143A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb

Plasmid

#238239

Purpose

For integration of mCherry_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry_P2AT2A_GFP-nb

Expression

Mammalian

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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