164,909 results

1. qTAG-N-Blast-dTAG-LMNB1

   Plasmid

   #207776

   Purpose

   Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. qTAG-C-mScarlet-Puro

   Plasmid

   #207722

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHR-CD28-mCherry

   Plasmid

   #223593

   Purpose

   Lentiviral expression of human CD28 with mCherry tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-GFP-Aurora B WT

   Plasmid

   #184042

   Purpose

   Expresses H2B-GFP fused to Aurora B WT

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Use

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSNAP-tag (m) Vector

   Plasmid

   #101135

   Purpose

   Expression of protein of interest with SNAP-Tag in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Type

   Empty backbone

   Use

   Tags

   SNAP-tag (SNAP26m)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSW002-Pc-mTurquoise2

   Plasmid

   #108235

   Purpose

   Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   Pc promoter - mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for experession in P. fluorescens

   Promoter

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0041-RanCas13b crRNA backbone

   Plasmid

   #103852

   Purpose

   For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-RanCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. DRD1-Tango

   Plasmid

   #66268

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   DRD1 (DRD1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAIP1

   Plasmid

   #233685

   Purpose

   Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

   Depositor

   Christopher Bahl

   Insert

   Ulp1-like SUMO protease
   

   Use

   Tags

   CAHS-107838 and HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCEV-G2-Km ymNeongreen

    Plasmid

    #193959

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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