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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4881 - 4900 of 69471 results

  1. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  2. pIND-HA-ADH

    Plasmid
    #11991
    Depositor
    Steven Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    aldehyde dehydrogenase (ALDH1A1 Human)
    Use
    Ecdysone inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    no mutations
    Available since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only

  4. LDHA-HiBiT Vector

    Plasmid
    #238587
    Purpose
    Express LDHA-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has service
    20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA
    Insert
    LDHA (LDHA Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238587-plasmid-map-sequence-id-478465

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  6. pLenti6-DJ1-V5-C106D

    Plasmid
    #29413
    Depositor
    Mark Cookson
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Cys106Asp
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-CAG-Flex-Quetzal-S-LA-WPRE

    Plasmid
    #253388
    Purpose
    Enhanced dendritic spine (via LifeAct) localization of a Cre-dependent GCaMP8m-mScarlet fusion with a rigid covalent linker
    Depositor
    Ofer Yizhar
    Insert
    Quetzal-S-LA
    Use
    AAV
    Expression
    Mammalian
    Available since
    May 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    253388-plasmid-map-sequence-id-510503

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  10. mCherry-EB3-C-20

    Plasmid
    #55038
    Purpose
    Localization: MT End Binding Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3 (MAPRE3 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55038-plasmid-map-sequence-id-298517

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  11. SOD1-VC

    Plasmid
    #89758
    Purpose
    expresses human SOD1 fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Publication
    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SOD1 (SOD1 Human)
    Tags
    C-terminal part of Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    89758-plasmid-map-sequence-id-178833

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  13. R777-E162 Hs.PEBP1-nostop

    Plasmid
    #70446
    Purpose
    Gateway ORF clone of human PEBP1 [NM_002567.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PEBP1 (PEBP1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70446-plasmid-map-sequence-id-125895

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  14. pMAL-AGR2

    Plasmid
    #18097
    Depositor
    Ronny Drapkin
    Insert
    AGR2 (AGR2 Human)
    Tags
    MBP
    Expression
    Bacterial
    Available since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  16. pαH-S-RRAR/XBB.1.16

    Plasmid
    #213037
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact
    Depositor
    Priyamvada Acharya
    Publication
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    s -RRAR.XBB.1.16 (S )
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 furin site RRAR …
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213037-plasmid-map-sequence-id-423206

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  17. pαH-S-GSAS-D614G-deltaFV

    Plasmid
    #171743
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171743-plasmid-map-sequence-id-337714

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  19. pC-HYG-YR

    Plasmid
    #61765
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Publication
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61765-plasmid-map-sequence-id-112551

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  20. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid
    #183502
    Purpose
    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.
    Depositor
    Wayne Tilley
    Publication
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    mKate2
    BSD
    Use
    Lentiviral and Luciferase
    Tags
    P2A linker and SV40 NLS with GGS linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    183502-plasmid-map-sequence-id-362519

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Showing: 4881 - 4900 of 69471 results