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We narrowed to 7,494 results for: tet on

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Showing: 4921 - 4940 of 7494 results

  9. pLA2ScIfmTcIfm

    Plasmid
    #193792
    Purpose
    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLLacO-1 and PLTetO-1
    Available Since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    193792-plasmid-map-sequence-id-390100

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  10. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid
    #169735
    Purpose
    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169735-plasmid-map-sequence-id-334142

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  11. ARB366

    Plasmid
    #124049
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124049-plasmid-map-sequence-id-252514

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  12. ARB367

    Plasmid
    #124050
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124050-plasmid-map-sequence-id-252515

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  14. pAN1646

    Plasmid
    #127205
    Purpose
    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    Use
    Synthetic Biology
    Availability
    Academic Institutions and Nonprofits only
    127205-plasmid-map-sequence-id-250026

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  15. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Available Since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

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  18. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    tetO promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183766-plasmid-map-sequence-id-361683

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Showing: 4921 - 4940 of 7494 results