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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,915 results for: SPI

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Showing: 4921 - 4940 of 5915 results

  2. pEK42

    Plasmid
    #214958
    Purpose
    Endogenous tagging of B. dendrobatidis GAPDH with a red fluorescent protein, hygromycin B selection
    Depositor
    Lillian Fritz-Laylin
    Article
    Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122
    Inserts
    BdGAPDH_LHA2-G4S-HsmRuby3-ScADH1ter
    SpH2Bpro-Hshph-BdGAPDH_RHA2
    Use
    Bd gene targeting vector
    Expression
    Bacterial
    Promoter
    SpH2Bpro
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    214958-plasmid-map-sequence-id-426037

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  3. pEK48

    Plasmid
    #214964
    Purpose
    Endogenous tagging of B. dendrobatidis Myo17D with a red fluorescent protein, hygromycin B selection
    Depositor
    Lillian Fritz-Laylin
    Article
    Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122
    Inserts
    BdMyo17D_LHA-G4S-HsmRuby3-ScADH1ter
    SpH2Bpro-Hshph-BdMyo17D_RHA
    Use
    Bd gene targeting vector
    Expression
    Bacterial
    Promoter
    SpH2Bpro
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    214964-plasmid-map-sequence-id-426034

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  10. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid
    #222876
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FRB chain: V84R in GPA
    Promoter
    MSCV LTR
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222876-plasmid-map-sequence-id-441621

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  19. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid
    #201024
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201024-plasmid-map-sequence-id-394447

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Showing: 4921 - 4940 of 5915 results