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pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Expression

Mammalian

Mutation

G124I

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-A'Q-mito

Plasmid

#158766

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-'Q-mito

Plasmid

#158767

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGal3

Plasmid

#73080

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin-3 (LGALS3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) WT V5 His

Plasmid

#119287

Purpose

CIP2A WT V5 overexpression

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Tags

V5 His

Expression

Mammalian

Promoter

T7

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-2xFYVE

Plasmid

#140047

Purpose

Expresses GFP-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available Since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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GST PTBP1 isoform 4

Plasmid

#108594

Purpose

Recombinant GST tagged protein production

Depositor

Chris Smith

Article

Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

Insert

PTBP1 isoform 4 (PTBP1 Human)

Tags

GST

Expression

Bacterial

Mutation

isoform 4 (isoform a)

Promoter

tac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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OMM-long-CFAST10

Plasmid

#233598

Purpose

Expression of CFAST10 on the OMM membrane after a long linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Tags

palmitoylated EGFP

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NOD1-EGFP

Plasmid

#131206

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD1 (NOD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP-shTACC3

Plasmid

#59356

Purpose

Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-Lck-mCherry-ibARK-4x6T

Plasmid

#241243

Purpose

Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

DIO-Lck-mCherry-ibARK-4x6T

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP VARP

Plasmid

#42312

Depositor

Thierry Galli

Article

Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

Insert

VARP (ANKRD27 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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