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pVETL-Cmcs-Flag-Ataxin3Q22-C14A

Plasmid

#22119

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

Plasmid

#163979

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mKdm4d(H189A)-polyA

Plasmid

#61554

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (catalytic mutant)

Tags

flag

Expression

Mammalian

Mutation

changed Histidine 189 (of Kdm4d) to Alanine

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3-Puro

Plasmid

#172619

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag RPS6KB2

Plasmid

#20625

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KB2 (RPS6KB2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K8

Plasmid

#20521

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K8 (MAP3K8 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP2K7

Plasmid

#20516

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K7 (MAP2K7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBIFC-flag-hCaspase 2 prodomain-VN173

Plasmid

#49262

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pSLIK 3XFLAG-EGFP-NLS hygro

Plasmid

#87781

Purpose

Inducible expression of EGFP with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag RET

Plasmid

#20614

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RET (RET Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

R982C

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-LC3B Q116P G120

Plasmid

#129289

Purpose

Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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His-SUMO- flag-hDDI2-D252N_pET11a

Plasmid

#169123

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Mutation

D252N

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CKMT1A

Plasmid

#20459

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKMT1A (CKMT1A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-hphMX-tetO-Pcyc1-3xFLAG

Plasmid

#41020

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-3xFLAG-MyD88-V5

Plasmid

#131351

Purpose

Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88 (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag AAK1

Plasmid

#20133

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AAK1 (AAK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pVETL-Cmcs-Flag-Ataxin3Q80-C14A

Plasmid

#22121

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pLC-Flag-CSNK1A1 G40N-Puro

Plasmid

#123320

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CDK2

Plasmid

#20447

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK2 (CDK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Y276C

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-FLAG

Plasmid

#162112

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-HA-FLAG

Use

Lentiviral

Tags

StrepTagII-HA-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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