We narrowed to 11,777 results for: 110

1. pCDNA3-Flag-SPRTN-Y117C

   Plasmid

   #110216

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y117C, P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Calprotectin S100A9 toehold switch sensor

   Plasmid

   #110717

   Purpose

   Toehold switch sensor to detect Calprotectin S100A9 mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   Calprotectin S100A9 toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-P5CR

   Plasmid

   #110295

   Purpose

   Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Pyrroline-5-carboxylate Reductase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.puro_shHuR 3UTR

   Plasmid

   #110414

   Purpose

   TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA PolIII)

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCHR-CD5Flag2-P2A-smAID-mClover

   Plasmid

   #110657

   Purpose

   Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CD5Flag2-P2A-AID-mClover
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM60 FLAG NUFIP1 W40A

   Plasmid

   #110951

   Purpose

   expresses mammalian FLAG-NUFIP1 W40A

   Depositor

   David Sabatini

   Article

   Wyant et al Science. 2018 May 18;360(6390):751-758.

   Insert

   NUFIP1 W40A (NUFIP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Tryptophan 40 to alanine

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW8_6XFLAG

   Plasmid

   #110235

   Purpose

   Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope

   Depositor

   Tony Carr

   Article

   Watson et al Gene. 2008 Jan 15;407(1-2):63-74

   Type

   Empty backbone

   Use

   Cre/Lox

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_GNAO1_G-alpha

   Plasmid

   #110013

   Purpose

   Protein expression and purification of GNAO1_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAO1_G-alpha (GNAO1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_HLA-DRB1_MHC-II-beta

   Plasmid

   #110021

   Purpose

   Protein expression and purification of HLA-DRB1_MHC-II-beta

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   HLA-DRB1_MHC-II-beta (HLA-DRB1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE hygro MEN1 T344R

    Plasmid

    #11019

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 T344R (MEN1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    T344R

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 HRPT2 L64P

    Plasmid

    #11052

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 L64P (CDC73 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L64P

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pKOdeltagalK (Apr)

    Plasmid

    #110091

    Purpose

    Analogous to pKO but without the negative selection gene galK.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKOdeltasacB (Kan)

    Plasmid

    #110092

    Purpose

    Analogous to pKO but without the negative selection gene sacB.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. plko-tet-on-puromycin-shTRX1-259

    Plasmid

    #110920

    Purpose

    To knockdown Thioredoxin-1 following doxycycline addition

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Lentiviral

    Promoter

    unknown

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE.puro/TO/Flag/tGFP.HuR.S221D

    Plasmid

    #110351

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Mutation

    S221D, P237S

    Promoter

    gag

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-PLEKHS1

    Plasmid

    #110509

    Purpose

    Fluorescent PLEKHS1

    Depositor

    Bernardo Mainou

    Insert

    PLEKHS1 (C10orf81 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHKU38.2-2.2

    Plasmid

    #110670

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
    

    Expression

    Yeast

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK103

    Plasmid

    #110582

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Lac promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 HRPT2 227X

    Plasmid

    #11050

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 227X (CDC73 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only