We narrowed to 4,109 results for: 272

1. pcDNA-PU.1-ETS N236Y

   Plasmid

   #124614

   Purpose

   Chimeric ETS domain: N236Y

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 236 altered from asparagine to a Tyrosine

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28B-PU.1-ETS-S3

   Plasmid

   #124550

   Purpose

   Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 242 to 246 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-PU.1-ETS-H2

   Plasmid

   #123358

   Purpose

   Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 207 to 215 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGK2 gRNA (BRDN0001145595)

   Plasmid

   #76946

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PGK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PGK2 (PGK2, PGK1P2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK2 gRNA (BRDN0001149179)

   Plasmid

   #76947

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PGK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PGK2 (PGK2, PGK1P2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PGK2 gRNA (BRDN0001146354)

   Plasmid

   #76948

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PGK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PGK2 (PGK2, PGK1P2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST40-ARL4D-V5-His6

   Plasmid

   #67447

   Purpose

   Mammalian expression of hArl4D with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET-DEST42-ARL4D-V5-His6

   Plasmid

   #67426

   Purpose

   Expression of hArl4D with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST47-ARL4D-GFP

   Plasmid

   #67405

   Purpose

   Mammalian expression of hArl4D with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. KHBD00003

    Plasmid

    #34166

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6272 (Irbp18 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. 289aa Met133, 138, 186Ileu ELL2

    Plasmid

    #127272

    Purpose

    Expresses 289aa human ELL2 with Met133, 138, 186Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met133, 138, 186 Ileu, cut with XbaI blunt fillin

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Myc var1 K51/52 to NQ

    Plasmid

    #127287

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K51 and 52 to N and Q by Q5 mutagenesis

    Promoter

    CMV

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Myc var1 K397R

    Plasmid

    #127285

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K397R by Q5 mutagenesis

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Myc var1

    Plasmid

    #127284

    Purpose

    Expresses Myc to see if degraded by ELLs

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    transcript variant 1

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Myc var 1 K396/397 to NQ

    Plasmid

    #127286

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K396 and 397 to N and Q by Q5 mutagenesi

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid

    #227273

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1+DPP6

    Plasmid

    #117272

    Purpose

    Encodes human DPP6 variant 1

    Depositor

    Mark Cannell

    Article

    Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

    Insert

    DPP6 (DPP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid

    #227275

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge