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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)
Plasmid#226989PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2bDepositorInsertSpCas9 gRNA 1a to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)
Plasmid#226991PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1bDepositorInsertSpCas9 gRNA 2a to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)
Plasmid#226987PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)DepositorInsertSpCas9 gRNA A6 to create OPA1-R194G
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)
Plasmid#226986PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)DepositorInsertSpCas9 gRNA A7 to create OPA1-R194G
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)
Plasmid#226992PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1bDepositorInsertSpCas9 gRNA 2b to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)
Plasmid#226990PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2bDepositorInsertSpCas9 gRNA 1b to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)
Plasmid#226988PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)DepositorInsertSpCas9 gRNA A9 to create OPA1-R194G
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pERB130
Plasmid#58284Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1DepositorInsertsMis12-GFP-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA
UseTagsGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB127
Plasmid#58283Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)DepositorInsertsMis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA
UseTagsGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB160
Plasmid#58285Purposeconst. Mis12-FKBP3; ind. FRB-mCH-Mad1DepositorInsertsMis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB122
Plasmid#58282Purposeconst: miRFKBP5_GFP_IRES_Puro; ind: mC-FRBDepositorInsertsFKBP-targeted miRNA
GFP
Puromycin resistance
inducible mCherry-FRB
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB171
Plasmid#58289Purposeconst. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718DepositorInsertsMis12-FKBPx3
inducible FRB-mCh-Mad1^485-718
FKBP-targeted miRNA
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB170
Plasmid#58288Purposeconst. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-584DepositorInsertsMis12-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMAL-CellTag-V1
Pooled Library#115643PurposeViral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamicsDepositorHas ServiceConcentrated Lentiviral PrepExpressionMammalianSpeciesSyntheticUseLentiviralAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMAL-CellTag-V2
Pooled Library#115644PurposeViral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamicsDepositorHas ServiceConcentrated Lentiviral PrepExpressionMammalianSpeciesSyntheticUseLentiviralAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMAL-CellTag-V3
Pooled Library#115645PurposeViral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamicsDepositorHas ServiceConcentrated Lentiviral PrepExpressionMammalianSpeciesSyntheticUseLentiviralAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Strain SIJ_488
Bacterial Strain#68246PurposeE. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.DepositorBacterial ResistanceNoneAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1a-Puro-V8.2
Pooled Library#178943PurposeBarcoding library for the macsGESTALT inducible lineage recording systemDepositorExpressionMammalianAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only
