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pDONR221 CARD8 FIIND L370G

Plasmid

#169970

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

L370G

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND L368G

Plasmid

#169969

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

L368G

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM L368G

Plasmid

#169985

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, L368G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM R394E

Plasmid

#169987

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, R394E

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F405G

Plasmid

#169988

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F405G

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F370G

Plasmid

#169986

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R

Plasmid

#169967

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E

Plasmid

#169971

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G

Plasmid

#169973

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND S297A

Plasmid

#169966

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

S297A

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Y365A or A365Y inducible rescue

Plasmid

#131323

Purpose

gRNA to generate i-WT-r or i-MT-r systems

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Y365A or A365Y inducible rescue

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666E

Plasmid

#105313

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666A

Plasmid

#105309

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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XE5 Xwnt-5A Hind RNA

Plasmid

#16872

Depositor

Randall Moon

Article

Moon et al Development. 1993 Sep. 119(1):97-111.

Insert

Xwnt-5A Hind RNA (wnt5a.S Frog)

Use

Xenopus expression

Mutation

Starts with AA 14 (see comments)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

Plasmid

#160540

Purpose

Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Inserts

SARS-CoV-2 NSP7 (ORF1ab Synthetic)
SARS-CoV-2 NSP8 (ORF1ab Synthetic)
SARS-CoV-2 NSP12 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MG15-luc (mut p53 binding sites)

Plasmid

#16443

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Promoter

hGFAP

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

Plasmid

#97044

Purpose

Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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SS13 lac-inducible GFP reporter circuit

Plasmid

#30336

Depositor

Herbert Sauro

Article

Sleight et al J Biol Eng. 2010 Nov 1;4:12

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only