We narrowed to 20,689 results for: Kars

1. pcDNA3-Golgi-deltaCKAR

   Plasmid

   #222428

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Golgi delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FluoSTEP-AKAR (C/Y)

   Plasmid

   #181862

   Purpose

   FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (C-Y)
   

   Use

   Tags

   CFP(1-10) and cpVenus(E127)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1C-Fluc-oskar_C

   Plasmid

   #146022

   Purpose

   Insect Expression of oskar-3UTR

   Depositor

   Elisa Izaurralde

   Insert

   oskar-3UTR (osk Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-lambdaN-HA-DmOskar_C

   Plasmid

   #146008

   Purpose

   Insect Expression of DmOskar

   Depositor

   Elisa Izaurralde

   Insert

   DmOskar (osk Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_KARS_tRNA-anti-codon

   Plasmid

   #109865

   Purpose

   Protein expression and purification of KARS_tRNA-anti-codon

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   KARS_tRNA-anti-codon (KARS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLAP-MIS12-KARD-3D

   Plasmid

   #114058

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   MIS12-KARD-3D (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   BUBR1-S670D, S676D and T680D

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLAP-MIS12-KARD-3A

   Plasmid

   #114059

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   MIS12-KARD-3A (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   BUBR1-S670A, S676A and T680A

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRKAR1A gRNA (BRDN0001144984)

   Plasmid

   #77721

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKAR1A (PRKAR1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRKAR1A gRNA (BRDN0001146809)

   Plasmid

   #77722

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKAR1A (PRKAR1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PRKAR1B gRNA (BRDN0001146606)

    Plasmid

    #76838

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PRKAR1B gRNA (BRDN0001144765)

    Plasmid

    #76839

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PRKAR1B gRNA (BRDN0001147089)

    Plasmid

    #76840

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid

    #123333

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123329

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid

    #115698

    Purpose

    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    SSKar2-sfpHluorin-HDEL
    

    Use

    Tags

    ER retention signal containing HDEL and ER signal…

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B

    Plasmid

    #100160

    Purpose

    Expresses PRKAR2B

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    PRKAR2B (PRKAR2B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZL Neo Myr Flag PRKAR2A

    Plasmid

    #20601

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PRKAR2A (PRKAR2A Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. 413-Met25 Kar2 TorsinA GFP

    Plasmid

    #32119

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only