We narrowed to 7,815 results for: Lif

1. BCL9-BirA*

   Plasmid

   #109147

   Purpose

   To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   BCL9 (BCL9 Human)
   

   Use

   Tags

   BirA(R118G)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRK5-FLAG-USP13/5-6

   Plasmid

   #61748

   Purpose

   express N-terminally FLAG tagged human chimera USP13/5-6

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13/5-6 chimera (USP5, USP13 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   USP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRK5-FLAG-USP13/5-5

   Plasmid

   #61747

   Purpose

   express N-terminally FLAG tagged human chimera USP13/5-5

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13/5-5 chimera (USP5, USP13 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   USP5 (1-781aa) fused to USP13 (786-864aa); G2667A…

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRK5-FLAG-USP13/5-2

   Plasmid

   #61744

   Purpose

   express N-terminally FLAG tagged human chimera USP13/5-2

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13/5-2 chimera (USP5, USP13 USP13/5-2 chimera, Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   USP5 (1-409aa) fused to USP13 (421-864aa)

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-GTSE1(long)-His6

   Plasmid

   #69110

   Purpose

   For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   G2- and S-phase Expressed 1 (GTSE1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1.2-GS

   Plasmid

   #162770

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. B9L-BirA*

   Plasmid

   #109146

   Purpose

   To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   B9L (Bcl9l Mouse)
   

   Use

   Tags

   BirA(R118G) and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

   Plasmid

   #79036

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Lys 283 to Asp and Gln 287 to Asp

   Promoter

   polyhedrin

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. VEE-mScarlet3-IRES-EGFP-IRES-E3-NSs-L*

   Plasmid

   #242409

   Purpose

   Self-amplifying RNA (saRNA) E3-NSs-L* construct; co-expresses vaccinia virus E3, Toscana virus NSs, and Theiler’s virus L*; more comprehensive inhibition of dsRNA-sensing pathways than plasmid 242408.

   Depositor

   Ewan St. John Smith

   Article

   Lim et al bioRxiv 2024.09.24.614636

   Inserts

   mScarlet3
   IRES-EGFP
   IRES-E3-NSs-L*
   

   Use

   Mouse Targeting; Self-amplifying rna

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPSt_35Spr-GFP-t35S

    Plasmid

    #203592

    Purpose

    Fully assembled Plant STARR-seq plasmid with the 35S terminator.

    Depositor

    Stan Fields

    Article

    Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

    Inserts

    CaMV 35S promoter + Zm00001d041672 5'UTR
    eGFP
    CaMV 35S terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMKRQ_11_DExCon-mNeonGreen-R25

    Plasmid

    #179896

    Purpose

    Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB25, member RAS oncogene family (RAB25 Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-2B-Nkx2-5 Y191C

    Plasmid

    #98613

    Purpose

    Gateway entry vector for NKX2-5 Y191C

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 Y191C (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Point mutation Y191C

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV Puro DHFR.cHSF1

    Plasmid

    #134739

    Purpose

    Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202

    Promoter

    CMV

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8/GW/TOPO-ERF

    Plasmid

    #98626

    Purpose

    Gateway entry TOPO TA cloning vector ERF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ERF (Erf Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-flex-TVA

    Plasmid

    #69618

    Purpose

    Express recepter TVA for envA with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Insert

    Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. CLXSN 5myc-CDC6-mut

    Plasmid

    #109333

    Purpose

    express tagged Cdc6 mutant

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    CDC6 (CDC6 Human)
    

    Use

    Retroviral

    Tags

    5x Myc

    Expression

    Mammalian

    Mutation

    R56A, L59A, K81A, E82A, N83A

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA-gp78-FLAG RING mutant C356G H361A

    Plasmid

    #61751

    Purpose

    express C-terminally FLAG tagged human gp78

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C356G H361A; P179L, F262L, Y315C, and D346G (ple…

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pENTR-2B-Gata4

    Plasmid

    #98615

    Purpose

    Gateway entry vector for Gata4

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    Gata4 (Gata4 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLgw EcoDam-V5-SRF

    Plasmid

    #98602

    Purpose

    Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    SRF (Srf Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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