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Plasmid#24922DepositorInsertAtg5 (ATG5 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-TGFB1
Plasmid#164427PurposeAAV plasmid expressing TGFB1 in photoreceptorsDepositorInsertTgfb1 (Tgfb1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterhuman red opsinAvailable sinceNov. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-mCherry
Plasmid#164426PurposeAAV plasmid expressing mCherry in photoreceptorsDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterhuman red opsinAvailable sinceNov. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-TGFB2
Plasmid#164428PurposeAAV plasmid expressing TGFB2 in photoreceptorsDepositorInsertTgfb2 (Tgfb2 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterhuman red opsinAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-TGFB3
Plasmid#164429PurposeAAV plasmid expressing TGFB3 in photoreceptorsDepositorInsertTgfb3 (Tgfb3 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterhuman red opsinAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-dnHIF
Plasmid#206343PurposeAAV plasmid expressing dominant negative HIF1A in photoreceptorsDepositorInsertdominant negative hif1a
UseAAVTagsExpressionMutationdominant negative HIF1APromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-nt.Txnip(C247S)(1-301aa)
Plasmid#206354PurposeAAV plasmid expressing deltion and mutant TXNIP in photoreceptorsDepositorInsertTxnip (Txnip Mouse)
UseAAVTagsExpressionMutation(1-301aa), C247SPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-prRDH-CTS
Plasmid#86069PurposeA bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.DepositorInsertRetinol dehydrogenase (RDH8 Bovine)
UseTagsGSTExpressionBacterialMutationthe insert is 297-312 of RDH8 (Bos taurus)PromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
OpenLoopControl
Plasmid#59895Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTRDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPESTExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3 (human)
Plasmid#24920PurposeMammalian expression of LC3 fused to EGFPDepositorInsertLC3 (MAP1LC3B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP6m-N3-TPC2
Plasmid#80147PurposeGCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real timeDepositorInsertTPCN2 (TPCN2 Human)
UseTagsGCaMP6mExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
hEGFR-mEGFP
Plasmid#182878PurposeMammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFPDepositorInserthEGFR-mEGFP (EGFR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-LC3 (Atg8)
Plasmid#24919DepositorInsertLC3 (MAP1LC3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-RPE65
Plasmid#206346PurposeAAV plasmid expressing RPE65 in photoreceptorsDepositorInsertRpe65 (Rpe65 Mouse)
UseAAVTagsExpressionMutation450-Met (C57BL6 natural variation)Promoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-Txnip(C247S)
Plasmid#206341PurposeAAV plasmid expressing mutant TXNIP in photoreceptorsDepositorInsertTxnip (Txnip Mouse)
UseAAVTagsExpressionMutationC247SPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR
Plasmid#164569PurposeSARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)DepositorInsertSpike (S-RRAR variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-eGFP.PB/PTK
Plasmid#170540PurposeExpresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restoredDepositorInsertDelta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-Lrat
Plasmid#206345PurposeAAV plasmid expressing LRAT in photoreceptorsDepositorInsertLrat (Lrat Mouse)
UseAAVTagsExpressionMutationPromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
sTR060
Plasmid#177370PurposeTrigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.DepositorInsertTrigger RNA
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only