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pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (AAV8)

Viral Prep

#65417-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (#65417). In addition to the viral particles, you will also receive purified pAAV-hSyn-dF-HA-KORD-IRES-mCitrine plasmid DNA. hSyn-driven, Cre-dependent KORD expression with bicistronic mCitrine, for SALB-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Promoter

Syn

Tags

mCitrine

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only
pMOS007: PercevalHR ATP/ADP sensor (cytosolic)

Plasmid

#163061

Purpose

PercevalHR ATP/ADP sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226166

Purpose

For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

2xMyc-tdMCP-SNAPtag

Use

Lentiviral

Tags

2xMyc-tdMCP and EGFP-IRES-BlastR

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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miRFP670nano-pcDNA

Plasmid

#127443

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS023: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163060

Purpose

Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-Traditional CAR (anti-CD19)

Plasmid

#192115

Purpose

Lentiviral delivery of Traditional CAR(anti-CD19)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141D

Plasmid

#69293

Purpose

Gateway entry vector; single gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS008: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163045

Purpose

GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

Plasmid

#163047

Purpose

GCaMPer calcium sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMPer

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ142

Plasmid

#69294

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHSV-TK

Plasmid

#165801

Purpose

Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

HSV-TK

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163043

Purpose

QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS028: Mitycam calcium sensor (mitochondrial)

Plasmid

#163046

Purpose

Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCpfSA

Plasmid

#187890

Purpose

CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

Depositor

Quanjiang Ji

Article

Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRU41

Plasmid

#167663

Purpose

ENTRY vector for gRNA1 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU43

Plasmid

#167665

Purpose

ENTRY vector for gRNA3 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

Plasmid

#175529

Purpose

Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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