We narrowed to 6,021 results for: RIK

1. pYX233-DHFR

   Plasmid

   #163761

   Purpose

   Gal-inducible yeast expression of cytosolic DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   DHFR (Dhfr Mouse)
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

   Plasmid

   #127177

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Promoter

   None

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TPC2(N257A)-mCherry

   Plasmid

   #135186

   Purpose

   Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Mutation of asparagine257 to alanine

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-FLAG-TAPBPR

   Plasmid

   #135500

   Purpose

   Mammalian expression of FLAG-tagged TAPBPR

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   TAPBPR (TAPBPL Human)
   

   Tags

   Luminal FLAG epitope tag and Signal peptide from …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVAX1-cGAS

   Plasmid

   #196624

   Purpose

   Express mouse cGAS protein in mammalian cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   cGAS (Cgas Mouse)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Flag-Tet1 CD Mut

   Plasmid

   #72218

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain (cata…

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-Flag-mRIPK1-S321A

   Plasmid

   #115339

   Purpose

   Mammalian expression of Flag-mRIPK1-S321A

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321A

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Vamp3-pHluorin

   Plasmid

   #190152

   Purpose

   Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp3 Rat)
   

   Use

   AAV

   Tags

   superecliptic pHluorin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

   Plasmid

   #205442

   Purpose

   production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

   Depositor

   Jeremy Kay

   Article

   Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

   Insert

   Unc5c (Unc5c Mouse)
   

   Use

   AAV

   Tags

   3xHA

   Promoter

   mini-CMV (with MVM small intron)

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TPC2(E643A)-mCherry

    Plasmid

    #135187

    Purpose

    Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of glutamate 643 to alanine

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GABA(A) receptor subunit B3SE

    Plasmid

    #49171

    Purpose

    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

    Insert

    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    

    Tags

    myc, pHGFP (pHluorin), and thrombin cleavage site…

    Expression

    Mammalian

    Mutation

    GTG (V) to TGC (C)

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid

    #113949

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-852

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV mScarlet-LC3B

    Plasmid

    #200431

    Purpose

    Mammalian expression of fluorescently-tagged marker for autophagic vesicles

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    Map1lc3b (Map1lc3b Rat)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-His-mTET1CD

    Plasmid

    #81053

    Purpose

    bacterial expression of the catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCVpuro-Gcn5

    Plasmid

    #63706

    Purpose

    retroviral expression of mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. mCardinal-H1-10

    Plasmid

    #56161

    Purpose

    Localization: Nucleus/Histones, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    H1 (H1f0 Mouse)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pKDR-Lenti-CBh.6-SOD1

    Plasmid

    #188460

    Purpose

    For knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Promoter

    CBh

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-KIF1A*-strep

    Plasmid

    #120165

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 1A (Kif1a Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF1A: 1-405 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Flag-Tet2 CD Mut

    Plasmid

    #72220

    Purpose

    Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 2 (Tet2 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET2 catalytic domain (cata…

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pGST-DHFR Mm

    Plasmid

    #83073

    Purpose

    Expresses the full length DHFR Mm in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    2x DHFR (Dhfr Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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