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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,336 results for: nls

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Showing: 5001 - 5020 of 22336 results

  1. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  6. pPHR-mCherry-NLS-VP16

    Plasmid
    #103821
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Publication
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103821-plasmid-map-sequence-id-203431

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  8. E407V Nuclear Myosin I YFP

    Plasmid
    #60242
    Purpose
    Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities
    Depositor
    Primal de Lanerolle
    Publication
    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Myosin 1 (Myo1c Mouse)
    Tags
    EYFP and NLS
    Expression
    Mammalian
    Mutation
    E407V
    Available since
    Nov. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid
    #158160
    Purpose
    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-3xHA-miniTurbo-3XNLS
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158160-plasmid-map-sequence-id-309971

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  18. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139087-plasmid-map-sequence-id-273883

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  19. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70703-plasmid-map-sequence-id-127765

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Showing: 5001 - 5020 of 22336 results