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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,912 results for: sta

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Showing: 5001 - 5020 of 14912 results

  1. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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  3. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Publication
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. mTagBFP2-FYN-N-10

    Plasmid
    #55297
    Purpose
    Localization: Membrane Associated Kinase, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Publication
    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FYN (FYN Human)
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. VRK1

    Plasmid
    #39067
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    VRK1 (VRK1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation K34A, K35A, E3…
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pAG mKappa Anti-c-Myc [9E10]

    Plasmid
    #242956
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242956-plasmid-map-sequence-id-485246

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  13. pD3sE_SC12

    Plasmid
    #219720
    Purpose
    DV3 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Publication
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV3 sE SC12
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219720-plasmid-map-sequence-id-433924

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  14. TFORF2159

    Plasmid
    #143347
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BCL11B (BCL11B Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143347-plasmid-map-sequence-id-400332

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  16. H2B-LSSmGFP

    Plasmid
    #186539
    Purpose
    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSSmGFP
    Tags
    Human Histone 2B (D26G and V119I mutations)
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186539-plasmid-map-sequence-id-368219

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  18. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164423-plasmid-map-sequence-id-338872

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Showing: 5001 - 5020 of 14912 results