We narrowed to 17,881 results for: puromycin

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pLenti-FRA1

Plasmid

#188665

Purpose

Expresses murine FRA1 (Fosl1)

Depositor

Günter Schneider

Publication

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro

Plasmid

#192875

Purpose

Cre-dependent dual reporter

Depositor

Kazuhiro Oka

Insert

Nanoluc-P2A-copGFP

Use

Lentiviral

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAK_lentiguide_DR30_Puro

Plasmid

#134839

Purpose

Lentiviral expression CasRx guideRNA

Depositor

Michael Yaffe

Insert

CasRx guideRNA backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Dsup pLVX-Puro

Plasmid

#180635

Purpose

Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

Depositor

Christopher Mason

Publication

Westover et al bioRxiv 2020.11.10.373571

Insert

Dsup

Use

Lentiviral

Promoter

CMV

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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W118-1_hTGM2-V5

Plasmid

#180413

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Publication

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K

Plasmid

#189907

Purpose

Gateway destination clone of CDC42BPA (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDC42BPA (CDC42BPA Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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SadCas9 KRAB

Plasmid

#188504

Purpose

Expresses FLAG tagged SadCas9 KRAB

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/N580A

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 VPR

Plasmid

#188510

Purpose

Expresses FLAG tagged HiFi dCas9 VPR

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-AID-NTERM-GFP

Plasmid

#185763

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SFFV-SMASH-CTERM-GFP

Plasmid

#185766

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-AID-CTERM-GFP

Plasmid

#185768

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z18-PDGFR_EF1a_eGFP

Plasmid

#186304

Purpose

Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z18-PDGFR
eGFP

Tags

HA, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO‐puro‐2A‐mdx4cv‐GFP

Plasmid

#187053

Purpose

fluorescent reporter for adenine base editing of mdx4cv point mutation

Depositor

Renzhi Han

Publication

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

Plasmid

#186699

Purpose

Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Publication

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ11440

Plasmid

#183203

Purpose

pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE

Depositor

Stanley Qi

Publication

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

hyperdCas12 base editor

Use

CRISPR and Lentiviral

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.KAT5

Plasmid

#184647

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Publication

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mTAGBFP2 NES visual barcode

Plasmid

#158667

Purpose

tagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1_SPTLC1_C133W

Plasmid

#181921

Purpose

3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

Depositor

Christian Metallo

Publication

Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

Insert

Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)

Use

Lentiviral; Mammalian expression, , doxycycline i…

Expression

Mammalian

Mutation

cysteine at position 133 of the coding region of …

Promoter

tight TRE promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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