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pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212830

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212831

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN001T-N_START

Plasmid

#218058

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001T-N_START

Use

Cloning plasmid

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN006A-P_START

Plasmid

#218059

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P_START

Use

Cloning plasmid

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN001M-T_START

Plasmid

#218060

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001M-T_START

Use

Cloning plasmid

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINOH11-A10

Plasmid

#184278

Purpose

Expresses nanobody H11-A10 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-A10

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11-B5

Plasmid

#184279

Purpose

Expresses nanobody H11-B5 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-B5

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_11-H6

Plasmid

#184280

Purpose

Expresses nanobody H11-H6 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H6

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINH11-H4 Q98R_H100E

Plasmid

#184281

Purpose

Expresses nanobody H11-H4 Q98R_H100E in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H4 Q98R_H100E

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 61-410

Plasmid

#185334

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 61-610 only

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-Gfi1b

Plasmid

#193088

Purpose

constitutive expression of mouse Gfi1b in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

Gfi1b (Gfi1b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-N-ZF(287-342)

Plasmid

#193068

Purpose

doxycycline-inducible expression of mouse Gata2 without the N-terminal zinc finger (amino acids 287-342), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-N-ZF(287-342)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-C-ZF(343-379)

Plasmid

#193069

Purpose

doxycycline-inducible expression of mouse Gata2 without the C-terminal zinc finger (amino acids 343-379), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-C-ZF(343-379)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-NLS(380-440)

Plasmid

#193070

Purpose

doxycycline-inducible expression of mouse Gata2 without the nuclear localisation signal (amino acids 380-440), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-NSL(380-440)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-N-Terminal(1-235)

Plasmid

#193067

Purpose

doxycycline-inducible expression of mouse Gata2 without the N-terminal (amino acids 1-235), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-N-Terminal(1-235)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1TMSNKRS

Plasmid

#198323

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot4-dsRNAres_Q

Plasmid

#147339

Purpose

Insect Expression of DmNot4-dsRNAres

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

DmNot4-dsRNAres (CG31716 Fly)

Expression

Insect

Mutation

7 silent mutations and 5 non silent mutations G20…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph18_eGFP_CCR5TC

Plasmid

#187443

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph15_eGFP_CCR5TC

Plasmid

#187442

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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