We narrowed to 8,178 results for: gnal

1. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Venus-cpVenus-FLARE-CKAR

   Plasmid

   #123343

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-FLCN (C terminus)

   Plasmid

   #72297

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

   Insert

   FLCN C-terminus (FLCN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains only C terminus of FLCN as defined in PM…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

   Plasmid

   #51252

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 789,805,817,848 mutated to Ala

   Promoter

   EF1a

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRK5 beta4 1355T

   Plasmid

   #16038

   Depositor

   Filippo Giancotti

   Article

   Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1355T: amino acids 1-1355

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

6. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_Hygro(+)_GRHL1-L3F6H

   Plasmid

   #177765

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1puro-shKIBRA-A

   Plasmid

   #40888

   Depositor

   Paul Reynolds

   Article

   Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

   Insert

   shKIBRA-A (WWC1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP Kind2 S181E

   Plasmid

   #105312

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181E

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP Kind2 S181A

    Plasmid

    #105307

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1puro-shKIBRA-B

    Plasmid

    #40889

    Depositor

    Paul Reynolds

    Article

    Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

    Insert

    shKIBRA-B (WWC1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR3-FLAG-Gephyrin G

    Plasmid

    #68817

    Purpose

    Geph G domain expression in mamalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCS2-zfIl34

    Plasmid

    #168112

    Purpose

    Express zebrafish Il34, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

    Insert

    Il34 (il34 Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GET1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176985

    Purpose

    Mammalian expression vector encoding GET1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GET1 (GET1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMP108 Nanog-Venus-2a-mCherry

    Plasmid

    #159743

    Purpose

    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

    Depositor

    Sergi Regot

    Article

    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    Venus-2a-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223-GOLPH3

    Plasmid

    #21688

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. LIC-Z-YFP

    Plasmid

    #153544

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    Venus YFP

    Expression

    Mammalian

    Mutation

    with mCherry in LIC-Z replaced by YFP

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-FLAG-HA-CAD S1900A

    Plasmid

    #46240

    Purpose

    FLAG-HA-CAD with S1900 mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1900 changed to Alanine (S1900A)

    Promoter

    CMV

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only