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OPA1-isoform2

Plasmid

#70174

Purpose

Mammalian expression of hsOPA1 isoform 2

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 2 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2z/b

Plasmid

#47584

Depositor

Emanuel Strehler

Publication

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

pMCA2z/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
FLAG-Pol-II CC

Plasmid

#35180

Depositor

Benjamin Blencowe

Publication

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains C-terminal domain repeats (1-22)X2

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pSVE Grb2 myc 49L/203R

Plasmid

#26087

Depositor

Bob Weinberg

Publication

Egan et al Nature. 1993 May 6. 363(6424):45-51.

Insert

Grb2 49L/203R (GRB2 Human)

Tags

myc

Expression

Mammalian

Mutation

49L/203R

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pHelp

Plasmid

#227583

Purpose

Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

Depositor

Chengzhang Fu

Publication

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

guide RNA scaffold

Use

Cloning vector

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFUS_A2B

Plasmid

#43850

Depositor

Takashi Yamamoto

Publication

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pXP622

Plasmid

#26849

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Anti-Rho [IPI-1D4] (Recombinant Antibody)

Antibody

#218110-rAb

Purpose

Anti-Rhodopsin chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to TETSQVAPA sequence.

Depositor

Institute for Protein Innovation (IPI)

Publication

Molday et al Biochemistry. 1983 Feb 1;22(3):653-60. d

Recommended applications

Western Blot

Reactivity

Human

Source species

Rabbit

Isotype

IgG

Trial size

Not available to purchase

Available since

May 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits
pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Publication

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Tags

histag

Expression

Bacterial

Promoter

T7 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shSNAI1

Plasmid

#115467

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shSNAI1 (SNAI1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD10

Plasmid

#31332

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2797

Plasmid

#63639

Purpose

pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB2796

Plasmid

#63641

Purpose

pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLAX13 (CT#232)

Plasmid

#13918

Depositor

Cliff Tabin

Publication

Logan et al Methods. 1998 Apr . 14(4):407-20.

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLV.CMV.flex.hM3Dq.Cherry

Plasmid

#170360

Purpose

Cre-inducible lentiviral vector expressing hM3Dq

Depositor

Johan Jakobsson

Publication

Klawonn et al Immunity. 2021 Feb 9;54(2):225-234.e6. d

Insert

flex.hM3Dq.Cherry

Use

Lentiviral

Promoter

CMV

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM272 - MCS mosTI Pmlc-2_GFP target

Plasmid

#159834

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab4a-c-Myc

Plasmid

#79799

Purpose

Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Publication

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Caspase-8 (C360A)-pcw107-V5

Plasmid

#64632

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CASP8 (transcript variant B) (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article