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pCMV-AID-BLM-UGI-T2A-mTagBFP2

Plasmid

#221222

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-tadDE-PcrA M6-UGI

Plasmid

#221236

Purpose

Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

tadDE-PcrA M6-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

Plasmid

#221223

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

Plasmid

#61590

Purpose

Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

Depositor

David Komander

Article

Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-244 and aa 698-708.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTP261

Plasmid

#104166

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

1x Cysteine located at bps 493-495 in full sequen…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro-eIF4E

Plasmid

#33252

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

eIF4E (EIF4E Human)

Use

Retroviral

Tags

untagged

Expression

Mammalian

Mutation

unmodified

Promoter

SV40

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-tomato SOX2-sgRNA

Plasmid

#196190

Purpose

Editing human SOX2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2 (SOX2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro G3BP1-sgRNA

Plasmid

#196197

Purpose

Cas9 + gRNA expression to edit G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFP-BAF_L58R

Plasmid

#101776

Purpose

Expresses EGFP tagged mutant BAF (L58R) in human cells

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

L58R mutation

Promoter

EF1a

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pGM117

Plasmid

#220168

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

CISD1 (CISD1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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ATG-1929

Plasmid

#108712

Purpose

CBR2opt in pF4Ag expression vector with T7 and CMV promoter

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt

Expression

Bacterial and Mammalian

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

T7, CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA-UGI-T2A-mTagBFP2

Plasmid

#221224

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MultiMate 5 colors

Plasmid

#206266

Purpose

Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)

Use

Recombinant baculovirus production, cre acceptor…

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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H14_MBP_SUMO-Cys-linker-BAF

Plasmid

#101777

Purpose

Expresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Promoter

T5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-SSeCKs T751A

Plasmid

#107142

Purpose

full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs T751A (Akap12 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed threonine 751 to alanine

Promoter

gag

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCR2.1_HBB_N

Plasmid

#87847

Purpose

pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

Depositor

Marina Kleanthous

Article

Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

Insert

Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR ASVinsertion

Plasmid

#32066

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Insert duplicate A767-V769

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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