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pT3-EF1A-CCNE2

Plasmid

#162554

Purpose

Overexpression of CCNE2

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE2 (CCNE2 Human)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-NanoLuc-BRAF-CAT

Plasmid

#194772

Purpose

Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)

Tags

NanoLuc

Expression

Mammalian

Promoter

CMV51

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-BRAF-REG-Halo

Plasmid

#194771

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV51

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

Plasmid

#75082

Purpose

An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Nuc-eYFP

Use

AAV and Cre/Lox

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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tetO-HEY2

Plasmid

#170691

Purpose

doxycycline-inducible overexpression of HEY2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

Plasmid

#162618

Purpose

Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43

Plasmid

#162617

Purpose

Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG-FRA1

Plasmid

#188742

Purpose

expresses murine Fosl1 (Fra1) with N-terminal tag FKBP F36V (dTAG) for the dTAG system

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Tags

FKBP F36V tag (dTAG)

Expression

Mammalian

Promoter

hPGK promoter

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1 KBTBD4 R313PRR

Plasmid

#184626

Purpose

Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

R313PRR

Promoter

TRE promoter, Tet ON

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

Plasmid

#162614

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP43 M337V

Plasmid

#162612

Purpose

Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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LeGO-iG-CSF2RB(A455D)

Plasmid

#125751

Purpose

Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

Depositor

Dirk Heckl

Article

Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

Insert

CSF2RB(A455D)

Use

Lentiviral

Mutation

A455D, codon optimized

Promoter

SFFV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-pGK-nAChRb2(E61C)-IRES-GFP

Plasmid

#164777

Purpose

cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

Insert

nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

E61C

Promoter

pGK

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pCW57.1 KBTBD4 P311PP

Plasmid

#184625

Purpose

Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 P311PP expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal Flag tagged KBTBD4 P311PP (KBTBD4 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

P311PP

Promoter

TRE promoter, Tet ON

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 P311PP

Plasmid

#184628

Purpose

Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)

Expression

Mammalian

Mutation

P311PP

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 R312RG

Plasmid

#184630

Purpose

Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)

Expression

Mammalian

Mutation

R312RG

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-DMD53-GFP

Plasmid

#138353

Purpose

Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences

Depositor

Baisong Lu

Article

Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do

Insert

DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

EF2

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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