We narrowed to 10,294 results for: otos
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Plasmid#224522PurposeA Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassetteDepositorInsertlox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
UseCre/LoxAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTph2p(330bp)-Luc
Plasmid#223663PurposeFirefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoterDepositorInsertmTph2 promoter (Tph2 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianPromotermTph2 promoterAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-LSL-LRRC37B
Plasmid#220791PurposeTo express the human receptor LRRC37B (cre-dependant) in mammalian cells together with EGFPDepositorInsertLRRC37B (LRRC37B Human)
Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-LRRC37B-HA
Plasmid#220792PurposeTo express the human receptor LRRC37B HA-tagged in mammalian cells together with EGFPDepositorInsertLRRC37B (LRRC37B Human)
TagsHAAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pSM
Plasmid#215871PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInsert3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pCM
Plasmid#215869PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-749_HA-GD2-28z_CAR_TFAP4
Plasmid#207490PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-852_HA-GD2-28z_TFAP4-HA
Plasmid#207502PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-858_CD19-BBz_CAR_TFAP4
Plasmid#207503PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, CD19-BBz_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC3_5-5)-PGKpuroBFP-W
Plasmid#211997PurposeExpress gRNA against ZIC3 with puro and BFPDepositorInsertsgRNA targeting ZIC3 (ZIC3 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC3_5-3)-PGKpuroBFP-W
Plasmid#211996PurposeExpress gRNA against ZIC3 with puro and BFPDepositorInsertsgRNA targeting ZIC3 (ZIC3 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W
Plasmid#211988PurposeExpress gRNA against TRIM24 with puro and BFPDepositorInsertsgRNA targeting TRIM24 (TRIM24 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM24_5-4)-PGKpuroBFP-W
Plasmid#211989PurposeExpress gRNA against TRIM24 with puro and BFPDepositorInsertsgRNA targeting TRIM24 (TRIM24 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM25_5-2)-PGKpuroBFP-W
Plasmid#211990PurposeExpress gRNA against TRIM25 with puro and BFPDepositorInsertsgRNA targeting TRIM25 (TRIM25 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM25_v3_6-4)-PGKpuroBFP-W
Plasmid#211991PurposeExpress gRNA against TRIM25 with puro and BFPDepositorInsertsgRNA targeting TRIM25 (TRIM25 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NR5A2_5-2)-PGKpuroBFP-W
Plasmid#211976PurposeExpress gRNA against NR5A2 with puro and BFPDepositorInsertsgRNA targeting NR5A2 (NR5A2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NR5A2_5-5)-PGKpuroBFP-W
Plasmid#211977PurposeExpress gRNA against NR5A2 with puro and BFPDepositorInsertsgRNA targeting NR5A2 (NR5A2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(PDLIM1_5-1)-PGKpuroBFP-W
Plasmid#211978PurposeExpress gRNA against PDLIM1 with puro and BFPDepositorInsertsgRNA targeting PDLIM1 (PDLIM1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(PDLIM1_Bru)-PGKpuroBFP-W
Plasmid#211979PurposeExpress gRNA against PDLIM1 with puro and BFPDepositorInsertsgRNA targeting PDLIM1 (PDLIM1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MYCN_5-3)-PGKpuroBFP-W
Plasmid#211972PurposeExpress gRNA against MYCN with puro and BFPDepositorInsertsgRNA targeting MYCN (MYCN Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MYCN_5-4)-PGKpuroBFP-W
Plasmid#211973PurposeExpress gRNA against MYCN with puro and BFPDepositorInsertsgRNA targeting MYCN (MYCN Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-MVP
Plasmid#204541PurposeExpresses GST-tagged MVP in E.coliDepositorAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-GFP
Plasmid#199220PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP
TagsEGFPExpressionMammalianPromoterCMV D2ir promoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
TagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…Available SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN687
Plasmid#177363PurposeExpresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tagDepositorInsertKIF1A (KIF1A Human)
TagsHis tag and Leucine zipperExpressionBacterialMutationchanged Proline 305 to LeucinePromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303C-hSOD1 A4V/C6S
Plasmid#139667PurposeExpression plasmids containing human A4V/C6S SOD1DepositorInsertSuperoxide dismutase-1 (SOD1 Human)
ExpressionBacterialMutationchange alanine 4 to valine, change cysteine 6 to …PromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA T7-Gnat1 Mut-3UTR
Plasmid#184027PurposeExpresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGADepositorInsertGnat1 (Gnat1 Mouse)
TagsT7ExpressionMammalianMutationTAG sites in the 3'-UTR are mutated to TGAPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-M135R-EGFP
Plasmid#164944PurposeTol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135RDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPMutationStop codon deleted; M135RPromoteractinb (actb2) promoterAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
KLC1N343S-Myc
Plasmid#166963PurposeExpresses Myc-tagged KLC1 N343S protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP
Plasmid#164945PurposeTol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFPDepositorInsertKCNJ13 (KCNJ13 Human)
UseTol2 transposon destination vectorTagsIRES-EGFPMutationStop codon deletedPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only