We narrowed to 9,851 results for: otos

1. AAV-CAG-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #105834

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DHC1-AID-Clover donor (Neo)

   Plasmid

   #158621

   Purpose

   Donor plasmid for tagging DHC1 with AID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR; Tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-KIF5A tail only

   Plasmid

   #166956

   Purpose

   Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   KIF5A (820-1027aa)

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GST-FUS 465-526

   Plasmid

   #44983

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS (465-526aa) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acids 465-526

   Promoter

   tac

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. GST-FUS 266-526

   Plasmid

   #44981

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS (266-526aa) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acids 266-526

   Promoter

   tac

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. GST-FUS 356-526

   Plasmid

   #44982

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS (356-526aa) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acids 356-526

   Promoter

   tac

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

   Plasmid

   #211986

   Purpose

   Express gRNA against SOX2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting SOX2 (SOX2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

   Plasmid

   #211987

   Purpose

   Express gRNA against SOX2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting SOX2 (SOX2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Epac-S-H94

   Plasmid

   #170341

   Purpose

   GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

    Plasmid

    #176892

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A-c-Myc
    

    Use

    Transposon

    Tags

    Myc and moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIG-836_NY-ESO-1_TCR_FAS/OX40

    Plasmid

    #207499

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/OX40, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Syn-FLEX-fd [Chronos-GFP]

    Plasmid

    #84482

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIG-833_NY-ESO-1_TCR_FAS/CD28

    Plasmid

    #207496

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/CD28, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIG-874_HA-GD2-28z_CAR_IL2RA

    Plasmid

    #207504

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    IL2RA, HA-GD2-28z_CAR (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

    Plasmid

    #208554

    Purpose

    Lentiviral vector expressing gRNA targeting human BCL11B-E1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B-E1(14) (BCL11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP.mIKKα

    Plasmid

    #74413

    Purpose

    Expresses mouse eGFP-IKKα in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    mouse IKKα (Chuk Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVZ_PcoaT_SQE1_LUP1

    Plasmid

    #103857

    Purpose

    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803

    Depositor

    Ilka Axmann

    Article

    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

    Insert

    lupeol synthase 1 (LUP1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    inserted genes are codon optimized. 2nd amino aci…

    Promoter

    PcoaT (from Synechocystis sp. PCC 6803)

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVTHM-Bptf-sh28

    Plasmid

    #83278

    Purpose

    shRNA targeting BPTF

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #28 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE-H1

    Available since

    Oct. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLVTHM-Bptf-sh5

    Plasmid

    #83277

    Purpose

    shRNA targeting BPTF

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #5 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE-H1

    Available since

    Oct. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Anti-NSD1 [N312/10]

    Plasmid

    #190311

    Purpose

    Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-EF1α1.1-Chronos-tdTomato

    Plasmid

    #122099

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPICZc-Ub-R-HsACTB*-thymosinB-8His

    Plasmid

    #111149

    Purpose

    Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration plasmid

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    N-terminal arginylation

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-PGK-DIO-mCherry-nega miR

    Plasmid

    #137732

    Purpose

    Lentivirus vector for control miR in a Cre-dependent manner

    Depositor

    Tomoyuki Furuyashiki

    Article

    Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

    Insert

    control microRNA
    

    Use

    Lentiviral

    Tags

    mChherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid

    #122103

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #108273

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α promoter (1.1kb short version)

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

    Plasmid

    #84485

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α, 1.1 kb long (short version)

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICE-EGFP-FLAG-TK1

    Plasmid

    #161919

    Purpose

    Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Thymidine kinase, cytosolic (TK1 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSIREN-RetroQ-BPTF-sh3

    Plasmid

    #73667

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to BPTF

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #3 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

    Plasmid

    #118295

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge