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pLX304 PCSK5 (H516P)-V5 blast

Plasmid

#83103

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Histidine 516 to Proline

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCHCS163.2-2.2

Plasmid

#115527

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Mutation

a mutated form of FLP, which at position 5 contai…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU16.1-2.2

Plasmid

#115529

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU20.1-2.2

Plasmid

#115531

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU11.1-2.2

Plasmid

#115528

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU71.2-2.2

Plasmid

#110685

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU68.2-2.2

Plasmid

#110681

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU27.2-2.2

Plasmid

#110662

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU75.2-2.2

Plasmid

#110691

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU70.2-2.2

Plasmid

#110683

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU33.2-2.2

Plasmid

#110667

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU32.2-2.2

Plasmid

#110665

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU30.2-2.2

Plasmid

#110663

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

Plasmid

#101210

Purpose

Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

Plasmid

#101203

Purpose

Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

Plasmid

#101212

Purpose

Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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KC1G3_HUMAN_D0

Plasmid

#79685

Purpose

This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G3 (CSNK1G3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2_3xflag_CHEK2

Plasmid

#136526

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CHEK2 (CHEK2 Human)

Use

Gateway Cloning

Tags

3x FLAG

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.1_puro_shJUND #2

Plasmid

#136583

Purpose

Expresses an inducible short hairpin targeting human JUND sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shJUNDv2 (JUND Human)

Use

Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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