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pCMV NucleoBAS

Plasmid

#62861

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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409 pSG5L HA RB (379-928)

Plasmid

#10734

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (379-928) (RB1 Human)

Expression

Mammalian

Mutation

amino acids 379-928

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCI-hFADD-FLAG

Plasmid

#31814

Depositor

Astar Winoto

Article

Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

Insert

FADD (FADD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

Plasmid

#71282

Purpose

Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Promoter

HSP70l

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Synthetic, Budding Yeast, Human)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL4C-GFP

Plasmid

#67402

Purpose

Mammalian expression of hArl4c with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
mCardinal-H2B-C-10

Plasmid

#56162

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H2B (H2BC11 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
608 pSG5L HA RB del ex4

Plasmid

#10730

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del exon4 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of exon4

Available Since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
mCardinal-Tubulin-C-18

Plasmid

#56176

Purpose

Localization: Microtubules, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tubulin (TUBA1B Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
498 pSG5L HA RB del663 (NAAIRS)

Plasmid

#10728

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del663 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

residues 664 to 669 were replaced by NAAIRS, a fl…

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pDEST47-SARA-GFP

Plasmid

#67410

Purpose

Mammalian expression of hSara with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGEX5X.1 PINK1 KD

Plasmid

#13322

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496; Kinase dead: K219D/D362A/D3…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pGL3-DICER-Prom

Plasmid

#25851

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

Dicer promoter (DICER1 gene ID 23405, Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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mCardinal-PDHA1-N-10

Plasmid

#56172

Purpose

Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

PDHA1 (PDHA1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDRIVE-CAG-hFX-HA

Plasmid

#14994

Depositor

Michael Ristow

Article

Schulz et al Aging (Albany NY). 2010 Nov;2(11):843-53

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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proE-cad670-Luc-mEbox

Plasmid

#42084

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -670 to …

Promoter

E-cadherin

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL1-GFP

Plasmid

#67395

Purpose

Mammalian expression of hArl1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 231 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBABE hygro MEN1 A242V

Plasmid

#11022

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A242V (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A242V

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only