We narrowed to 18,007 results for: puro

Showing: 5101 - 5120 of 18007 results

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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PB-3-FT-ENGRAM

Plasmid

#179158

Purpose

piggyBac plasmid for 3'-FT ENGRAM recorder

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Insert

Csy4

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

minP

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHBS1449 SED1-Lenti

Plasmid

#180824

Purpose

SED1 biosensor for stable integration and expression in mammalian cells

Depositor

Rajat Rohatgi

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 osmosensor

Use

Lentiviral

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149054)

Plasmid

#76712

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149112)

Plasmid

#76713

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM7 gRNA (BRDN0001147821)

Plasmid

#76112

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKD1 gRNA (BRDN0001149544)

Plasmid

#76841

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKD1 (PKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_Wild-type

Plasmid

#234551

Purpose

Human a-Ecat sequence/CTNNA1

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_Wild-type (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

None

Promoter

RSV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pJR107

Plasmid

#187245

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJR106

Plasmid

#187244

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-CTNNB1-ATG

Plasmid

#153429

Purpose

Expresses a gRNA that overlaps the startcodon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-epegRNA

Plasmid

#214100

Purpose

Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA/EGFR T790M epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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TNIK gRNA (BRDN0001146045)

Plasmid

#75849

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNIK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TNIK (TNIK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR3c-WT

Plasmid

#45711

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001145369)

Plasmid

#76141

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV CMV mCherry-DDX21-V5

Plasmid

#175163

Purpose

Lentiviral expression of human DDX21-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

DDX21 (DDX21 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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TBK1 gRNA (BRDN0001148607)

Plasmid

#76363

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSELECT-HA-mFOXO1

Plasmid

#83308

Purpose

Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection marker

Depositor

Steven Abcouwer

Article

Kong et al Exp Eye Res. 2016 Aug 7;151:82-95. doi:

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

hEF1/HTLV prom

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145694)

Plasmid

#80191

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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