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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 4

Plasmid

#236621

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-2333

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254076

Purpose

Retroviral transfer vector for tTA expression, puromycin selection marker.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

tTA

Use

Retroviral

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G-puro_V5-LOV-Turbo2-myc-EGFP

Plasmid

#253793

Purpose

Express V5-LOV-Turbo2-myc-EGFP under TRE3G (tetracycline inducible) promoter

Depositor

Xinnan Wang

Article

Kwak et al bioRxiv [Preprint]. 2025 Dec 23:2025.12.

Insert

V5-LOV-Turbo2-myc-EGFP

Use

Lentiviral; Tetracycline inducible promoter

Tags

V5-LOV-Turbo2-myc-EGFP

Expression

Mammalian

Promoter

pTRE3G

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G-puro_V5-LOV-Turbo2-myc-MIRO1-WT

Plasmid

#253794

Purpose

Express V5-LOV-Turbo2-myc-Miro1-WT under TRE3G (tetracycline inducible) promoter

Depositor

Xinnan Wang

Article

Kwak et al bioRxiv [Preprint]. 2025 Dec 23:2025.12.

Insert

V5-LOV-Turbo2-myc-Miro1-WT (RHOT1 Human)

Use

Lentiviral; Tetracycline inducible promoter

Expression

Mammalian

Promoter

pTRE_tetracycline inducible

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G-puro_V5-LOV-Turbo2-myc-MIRO1-KK

Plasmid

#253795

Purpose

Express V5-LOV-Turbo2-myc-Miro1-KK under TRE3G (tetracycline inducible) promoter

Depositor

Xinnan Wang

Article

Kwak et al bioRxiv [Preprint]. 2025 Dec 23:2025.12.

Insert

V5-LOV-Turbo2-myc-Miro1-WT (RHOT1 Human)

Use

Lentiviral; Tetracycline inducible promoter

Expression

Mammalian

Promoter

pTRE_tetracycline inducible

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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ePB-Puro_TT-hCDX2-P2A-tdTomato-CAAX

Plasmid

#252249

Purpose

A PiggyBac donor vector for Dox-inducible expression of CDX2 and membrane-localized tdTomato.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

CDX2 (CDX2 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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ePB-Puro_TT-hGATA6-T2A-EGFP-CAAX

Plasmid

#252248

Purpose

A PiggyBac donor vector for Dox-inducible expression of GATA6 and membrane-localized EGFP.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

GATA6 (GATA6 Human)

Use

Piggybac transposon donor vector

Tags

T2A-EGFP-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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ePB-Puro_TT-hGATA3-P2A-tdTomato-CAAX

Plasmid

#252250

Purpose

A PiggyBac donor vector for Dox-inducible expression of GATA3 and membrane-localized tdTomato.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

GATA3 (GATA3 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 6

Plasmid

#236623

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1831-2663

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 2

Plasmid

#236619

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-1006

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1

Plasmid

#236618

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-658

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-WT

Plasmid

#242749

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCLJ-P2F-Frt-PGK-Puro-Frt

Plasmid

#227501

Purpose

Inserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Fgf5 promoter

Use

AAV and Mouse Targeting

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1-IRES-mCherry

Plasmid

#232239

Purpose

Mammalian expression of REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #1-gRNA-Cas9-Puro

Plasmid

#186892

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4

Plasmid

#216823

Purpose

Expresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8

Plasmid

#216824

Purpose

Expresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3

Plasmid

#216825

Purpose

Expresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-3

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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