We narrowed to 7,128 results for: SIM

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B

Plasmid

#103002

Purpose

non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Article

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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GW1-mito-roGFP1-mApple

Plasmid

#107218

Purpose

Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

roGFP1-mApple

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA HA DUSP6

Plasmid

#236430

Purpose

transient overexpression of DUSP6 in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

DUSP6 (DUSP6 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGRG36-Kn-PA1-GFP

Plasmid

#79088

Purpose

pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

Depositor

Frank Rosenzweig

Article

Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

Insert

GFP

Use

Bacterial chromosome integration and expression o…

Expression

Bacterial

Promoter

PA1

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mTLR4ecd-VLR

Plasmid

#187877

Purpose

Baculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

TLR4ecd (Tlr4 Mouse)

Expression

Insect

Mutation

includes residues 26–544 of mouse TLR4

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-ACEmax

Plasmid

#139105

Purpose

Mammalian Target-ACEmax expression vector (pSI-625)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

Plasmid

#159689

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

Depositor

Dominic Esposito

Article

Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-ArchT-tdTomato

Plasmid

#29778

Purpose

AAV-mediated expression of ArchT-tdTomato for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-tdTomato

Use

AAV; Adeno-associated virus

Tags

tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(AtUBI10)

Plasmid

#202016

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

AtUBI10

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-LRPAP1

Plasmid

#31327

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

low density lipoprotein receptor-related protein associated (LRPAP1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1

Plasmid

#224719

Purpose

For expression of HA-tagged GTSE1 protein

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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PM-RspA-CFAST

Plasmid

#233601

Purpose

Expression of RspA-CFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-IgKL-mNG

Plasmid

#183467

Purpose

To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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