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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 dUBA (1-389)

Plasmid

#210426

Purpose

Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL dUBA (1-389) (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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EndophilinA1-mRuby2

Plasmid

#171945

Purpose

Expresses Endophilin A1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Endophilin A1 (SH3GL2 Human)

Tags

mRuby2

Expression

Mammalian

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A

Plasmid

#24898

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A

Tags

GFP

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pCB6 ZO1myc

Plasmid

#30317

Depositor

James Anderson, Alan Fanning

Article

Fanning et al J Biol Chem. 1998 Nov 6. 273(45):29745-5

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

myc

Expression

Mammalian

Mutation

none

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
phU1-CasRx-non-targeting (pKW82)

Plasmid

#229050

Purpose

Cloning backbone for CasRx crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B

Plasmid

#121157

Purpose

Mammalian expression of GFP tagged TIS11B

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) (ZFP36L1 Human)

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo.mCherry-NEDD4

Plasmid

#38316

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

Nedd4 (NEDD4 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf1 C699A

Plasmid

#11753

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

C699A: catalytically inactive

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCZGY546

Plasmid

#90466

Purpose

Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-mcherry

Tags

mcherry

Expression

Worm

Promoter

Pmec-4

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHIV_pTp66p51

Plasmid

#78233

Purpose

Expresses HIV reverse transcriptase

Depositor

Christopher Voigt

Article

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Inserts

p66
p51
LacI

Expression

Bacterial

Promoter

Const. Promoter (From DNA2.0 Vec) and pTac Minima…

Available Since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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p006-GFP-strong

Plasmid

#108313

Purpose

GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

pFAB4026

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTW334i

Plasmid

#237319

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRN3P_Kdm5b_MUT

Plasmid

#119767

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

Kdm5b MUT

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMF19

Plasmid

#113662

Purpose

Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

Depositor

Miguel Valvano

Article

Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

Insert

wbbL

Promoter

Ptac

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p006-GFP-pBAD

Plasmid

#108315

Purpose

GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

GFP under control of arabinose-inducible promoter pBAD

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pOG231

Plasmid

#17736

Depositor

Geoff Wahl

Article

O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

Insert

NLS-Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pHen2 Avi-tag-linker-p3

Plasmid

#119819

Purpose

For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

linker with Avi-tag and p3

Tags

Avi-tag

Expression

Bacterial

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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