We narrowed to 8,464 results for: Dos;
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Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
TagsEGFP and mCherryExpressionMammalianAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 1-154 (XSB382)
Plasmid#78510PurposeConstruct for expressing Grb2 lacking the C-terminal SH3 domain in E. ColiDepositorInsertGrb2 1-154 (GRB2 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-3XFLAG
Plasmid#105784PurposeExpression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-3XFLAGExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mVenus
Plasmid#105791PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mVenusExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330 Rosa26 Gu1 (LH416)
Plasmid#99629PurposePlasmid used to target the wildtype Rosa26 allele with Cas9DepositorInsertCas9
UseCRISPRAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT3014
Plasmid#122540PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
TagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Fn
Plasmid#108864PurposeFnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processingDepositorTypeEmpty backboneUseCRISPR; Gateway compatible crrna entry cloneExpressionPlantAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv2
Plasmid#188604PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-MBP
Plasmid#105787PurposeExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-MBPExpressionMammalianAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdKatushka2-Fibrillarin-7
Plasmid#56041PurposeLocalization: Nucleoli, Excitation: 586, Emission: 632DepositorInsertFibrillarin (FBL Human)
TagstdKatushka2ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable SinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_LASP1_LIM
Plasmid#109780PurposeProtein expression and purification of LASP1_LIMDepositorInsertLASP1_LIM (LASP1 Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_V5-ANKRD52
Plasmid#92012PurposeExpresses V5-ANKRD52DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe JunD-HA neo
Plasmid#58486Purposemammalian expression of mouse JunDDepositorAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT1
Plasmid#73943PurposeXenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tagDepositorInsertPepT1 (SLC15A1 Human)
UseMake rna for xenopus oocyte injectionTagsFlagPromoterbeta globinAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagPromoterbeta globinAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_eGFP::Dpp
Plasmid#163702PurposeGFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancersDepositorAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1K908E-VSV
Plasmid#139357PurposeExpression of kinase-dead JAK1-K908E proteinDepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
TagsVSVExpressionMammalianMutationK908EPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV4-A
Plasmid#74975PurposeshETV4 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only