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YY105: pPB_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

Plasmid

#228882

Purpose

PiggyBac plasmid expressing the HaloTag-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3 and PuroR under the control of a UbC promoter.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

HaloTag-fused GEMINI(A)

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R377

Plasmid

#138247

Purpose

Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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prNFL-EGFP

Plasmid

#132605

Purpose

Expresses EGFP-tagged rat NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A8

Plasmid

#132177

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A8 (SLC39A8 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1a-Itprip-V5

Plasmid

#120241

Purpose

Expresses V5-tagged Itprip in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Itprip (ITPRIP Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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anxA9-GFP

Plasmid

#107198

Purpose

Expresses human annexin A9-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

Insert

annexin A9 (ANXA9 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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NET5L pEGFP-N2 (1155)

Plasmid

#62035

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

NET5L (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

long form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
TAL2261

Plasmid

#36700

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BMPR1A-TALEN_Right (BMPR1A Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
TAL2452

Plasmid

#36841

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

RUNX1-TALEN_Left (Runx1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2260

Plasmid

#36699

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BMPR1A-TALEN_Left (BMPR1A Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEAK10-His-Myc-Kal5

Plasmid

#25440

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal5

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
mCherryS131C

Plasmid

#48732

Purpose

Expresses mCherryS131C in E. coli cells

Depositor

Bradley Olsen

Article

Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

Insert

mCherryS131C

Tags

6xHis

Expression

Bacterial

Mutation

changed serine 131 to cysteine

Promoter

T5

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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p1DT (chicken beta 1 integrin)

Plasmid

#14066

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLM1110_LEU2

Plasmid

#168460

Purpose

Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

mRFP1

Use

Synthetic Biology; Yeast assembly vector for big-…

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-mCherry

Plasmid

#170519

Purpose

Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-mCherry

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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piRFP-FRB-PMP-C-10

Plasmid

#139316

Purpose

peroxisome-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP: MTOR(2021-2113):[GGSA]2QASNSAVSGLRSGSSGG:PXMP(2-195)

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only