We narrowed to 29,617 results for: PLE

1. C67m

   Plasmid

   #120981

   Purpose

   MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Bxb1 integrase - mScarlet-i - LAA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGPDluxAB(Ura)

   Plasmid

   #1119

   Depositor

   Susan Lindquist

   Article

   Singer et al Mol Cell 1998 Apr;1(5):639-48.

   Insert

   bacterial luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   n/a

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

3. Abr in pAcG2T

   Plasmid

   #38162

   Depositor

   Nora Heisterkamp

   Insert

   ABR (ABR Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. UC16m

   Plasmid

   #121038

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gQi Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. UC17m

   Plasmid

   #121039

   Purpose

   MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gV1 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C105m

   Plasmid

   #121008

   Purpose

   MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP-AAV ssrA (has bbsI)
   

   Use

   Synthetic Biology

   Tags

   AAV degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C106m

   Plasmid

   #121009

   Purpose

   MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP-DAS ssrA (has bbsI)
   

   Use

   Synthetic Biology

   Tags

   DAS degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. C109m

   Plasmid

   #121011

   Purpose

   MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mfLon protease with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Splicing-targeting CRISPR-Cas9 library for human lncRNAs

   Pooled Library

   #119977

   Purpose

   The CRISPR sgRNA library was designed to target the 5' or 3' splice sites of lncRNAs in genome-scale, in order to knockout the targeted lncRNAs.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

10. Human ABC Transporter Knockout Sub-library with UMIs

    Pooled Library

    #221410

    Purpose

    gRNA library to test the impact of transporters on biological processes (subset: ABC transporters and controls)

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pLIX_403

    Plasmid

    #41395

    Purpose

    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-mCardinal-SKL

    Plasmid

    #183728

    Purpose

    For exrpression of mCardinal targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mCardinal
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-RFP-SKL

    Plasmid

    #183726

    Purpose

    For expression of RFP targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mRFP1
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-tagged Bcr

    Plasmid

    #38189

    Depositor

    Nora Heisterkamp

    Insert

    BCR (BCR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18T-mini-Tn7T-Tp

    Plasmid

    #65024

    Purpose

    TpR mini-Tn7 vector, mobilizable

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGEX-4T-1-3xHA

    Plasmid

    #129567

    Purpose

    Empty backbone for HA tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50468

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG5-Crkl mut SH2/SH3

    Plasmid

    #41704

    Depositor

    Nora Heisterkamp

    Insert

    Crkl (CRKL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R39K and W160K

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pSG5-Crkl mut SH2

    Plasmid

    #41703

    Depositor

    Nora Heisterkamp

    Insert

    Crkl (CRKL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R39K

    Promoter

    SV40

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pENTR-flbio

    Plasmid

    #30216

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Insert

    No gene
    

    Use

    Gateway entry plasmid

    Tags

    Bio and Flag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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