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pcDNA3.1(+)-human LRRC33

Plasmid

#111602

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Publication

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

myc/his tag

Expression

Mammalian

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1(+)-human LRRC33

Plasmid

#111602

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Publication

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

myc/his tag

Expression

Mammalian

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::NLuc

Plasmid

#124533

Purpose

p53 NLuc luciferase reporter

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P53 NLuc reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::NLuc

Plasmid

#124533

Purpose

p53 NLuc luciferase reporter

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P53 NLuc reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Publication

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Expression

Bacterial

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Publication

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Expression

Bacterial

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#81021

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Publication

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#81021

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Publication

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Publication

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Publication

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Publication

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Publication

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ILF2_DZF_pGEX

Plasmid

#135162

Purpose

Recombinant expression of ILF2 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

ILF2 (ILF2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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ILF2_DZF_pGEX

Plasmid

#135162

Purpose

Recombinant expression of ILF2 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

ILF2 (ILF2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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