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pLVX-IRES-Gαq*-BERKY3

Plasmid

#158425

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Lentiviral

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Tags

HaloTag

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Tags

HaloTag

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-HCMVgO-sfGFP (TB40/E)

Plasmid

#136451

Purpose

Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

Depositor

Erik Procko

Publication

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein O (UL74 Human betaherpesvirus 5)

Tags

Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH (TB40/E)

Plasmid

#136446

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

Depositor

Erik Procko

Publication

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (1-513aa)

Plasmid

#75479

Purpose

mammalian expression of GADD34 1-513 aa

Depositor

Shirish Shenolikar

Publication

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 1-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(Trp1)

Plasmid

#49459

Purpose

overespression of Flp step-arrest mutant in yeast, TRP1 selection

Depositor

Marc Gartenberg

Publication

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pWZLblast-RalA WT(RalA 402i-resistant)

Plasmid

#11124

Depositor

Christopher Counter

Publication

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalA WT (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

resistant to siRNA 402i

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-ncpGCaMP6s

Plasmid

#113674

Purpose

A topological variant of GCaMP6s

Depositor

Robert Campbell

Publication

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

ncpGCaMP6s

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-deGFP-T500

Plasmid

#45392

Depositor

Vincent Noireaux

Publication

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

PL-tetO1

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCA-FLEX

Plasmid

#38041

Depositor

Naoshige Uchida

Publication

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

CA

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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ZE3 Wnt-8

Plasmid

#17044

Depositor

Randall Moon

Publication

Kelly et al Development. 1995 Jun. 121(6):1787-99.

Insert

wnt-8 full length (wnt8a Zebrafish)

Use

Zebrafish expression

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMJO-7

Plasmid

#31992

Depositor

Mark Osborn

Publication

Osborn et al Hum Gene Ther. 2011 Jun 13.

Type

Empty backbone

Expression

Mammalian

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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PAK4-FL-3'UTR

Plasmid

#113088

Purpose

Luciferase reporter containing PAK4- FL-3'UTR

Depositor

James Donahue

Publication

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(Trp1)

Plasmid

#49459

Purpose

overespression of Flp step-arrest mutant in yeast, TRP1 selection

Depositor

Marc Gartenberg

Publication

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pWZLblast-RalA WT(RalA 402i-resistant)

Plasmid

#11124

Depositor

Christopher Counter

Publication

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalA WT (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

resistant to siRNA 402i

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7g1(K165A)

Plasmid

#14675

Depositor

Roger Davis

Publication

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1(K165A) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Lys-165Ala

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only