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pKP2202

Plasmid

#167613

Purpose

Express ExbD V49A, P50A

Depositor

Kathleen Postle

Article

Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

Insert

exbD V49A, P50A

Expression

Bacterial

Mutation

exbD with V49A, LP50A mutation

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKP2201

Plasmid

#167611

Purpose

Express ExbD V47A, L49A

Depositor

Kathleen Postle

Article

Kopp et al J Bacteriol. 2020 Mar 11;202(7). pii: JB

Insert

exbD V47A, L49A

Expression

Bacterial

Mutation

exbD with V47A, L49A mutation

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKP920

Plasmid

#160814

Purpose

Expression of ExbB protein

Depositor

Kathleen Postle

Article

Ollis et al J Bacteriol. 2012 Jun;194(12):3069-77. d

Insert

exbB

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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CI-5

Plasmid

#135169

Purpose

Expressing cysJ-infA functional variant 5

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-5

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-6

Plasmid

#135168

Purpose

Expressing cysJ-infA functional variant 6

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-6

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-4

Plasmid

#135167

Purpose

Expressing cysJ-infA functional variant 4

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-4

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-3

Plasmid

#135166

Purpose

Expressing cysJ-infA functional variant 3

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-3

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-2

Plasmid

#135165

Purpose

Expressing cysJ-infA functional variant 2

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-2

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-1

Plasmid

#135164

Purpose

Expressing cysJ-infA functional variant 1

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-1

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHH01_32

Plasmid

#120892

Purpose

Selection of ECF for controller implementation

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Insert

ECF32_1122

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pIAGO-pegA + aceS

Plasmid

#114457

Purpose

Expression of PegA and AceS in streptomycetes

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

pegA + aceS

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-tvhc-DHFR

Plasmid

#116048

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tvhc-DHFR

Expression

Mammalian

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEV-teTVMV2

Plasmid

#116098

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEV-teTVMV2

Expression

Mammalian

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-tehc-DHFR

Plasmid

#116046

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-Cer-HO1-FlpIn

Plasmid

#116037

Purpose

Supporting plasmid for testing protease circuits

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cer-HO1

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-Cit-tehc-DHFR

Plasmid

#116058

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pWT047c

Plasmid

#107903

Purpose

W2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PR-sd5-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT060j

Plasmid

#107906

Purpose

W2m.0-4 plasmid. Contains U6-sgRNA C (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA C (CCR5)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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MSP850

Plasmid

#98797

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HPLVGHM-TEV L1F 111215

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP851

Plasmid

#98798

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HPLVGHM-TEV L2F 111215

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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