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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,557 results for: Flp

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Showing: 1001 - 1040 of 1557 results

  3. pVBL0001

    Plasmid
    #242429
    Purpose
    Stable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.
    Depositor
    Vladislav Belyy
    Article
    Smith et al bioRxiv [Preprint]. 2025 Jul 21:2025.07.
    Insert
    IRE1alpha (ERN1 Human)
    Tags
    mCherry, CRY2clust
    Expression
    Mammalian
    Mutation
    Deletion of the lumenal domain
    Promoter
    CMV, tet-inducible
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242429-plasmid-map-sequence-id-484493

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  4. EW428 CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deImmLink-ZIK1KRAB

    Plasmid
    #236141
    Purpose
    Plasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-ZIK1KRAB
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    236141-plasmid-map-sequence-id-471244

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  8. SCN1Aprom(1-500)-H2B-GFP

    Plasmid
    #236162
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 500 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236162-plasmid-map-sequence-id-468412

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  9. SCN1Aprom(335-406)-H2B-GFP

    Plasmid
    #236151
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236151-plasmid-map-sequence-id-468384

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  11. EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB

    Plasmid
    #236140
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236140-plasmid-map-sequence-id-468371

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  12. EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB

    Plasmid
    #236142
    Purpose
    Plasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB
    Expression
    Mammalian
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236142-plasmid-map-sequence-id-468373

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  22. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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  23. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  38. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  39. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid
    #52890
    Purpose
    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Inserts
    LexGAD
    Gal4 (GAL4 Budding Yeast)
    Expression
    Insect
    Available Since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52890-plasmid-map-sequence-id-211914

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Showing: 1001 - 1040 of 1557 results