Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

To test the effect of sequence on TDP43 splicing activity

dCas9 with single cysteine residue (M1C) for site-specific labelling

All-in-one plasmid targeting tdTomato

All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

Integration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

RLC11 pooled library

RLC12 pooled library

Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline

Fluorescently labelled (GFP) E. coli, auxotrophic for proline

Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.